Chicken CT ELISA Kit | CT elisa kit

Chicken CT (Calcitonin) ELISA Kit

Cat. Num. AAA2511712

• Gene Names: CALCA; CT; KC; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Chicken
• Synonyms: CT; Chicken CT (Calcitonin) ELISA Kit; CT elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes Chicken CT in samples. No significant cross-reactivity or interference between Chicken CT and analogues was observed.
• Sequence Length: 141

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 46.875pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Chicken CT were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Chicken CT were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CT elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CT concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken CT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Chicken CT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Chicken CT. You can calculate the concentration of Chicken CT in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502545
• NCBI GeneID: 796
• NCBI Accession #: NP_001732.1
• NCBI GenBank Nucleotide #: NM_001741.2
• UniProt Accession #: P01258; Q13935; Q13937; Q52LX7; B7ZL39; P06881
• Molecular Weight: 13,898 Da
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin-related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1; alpha-type CGRP; calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin
• UniProt Protein Name: Calcitonin
• Protein Family: Ctenidin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; leukocyte adhesion; elevation of cytosolic calcium ion concentration; cell-cell signaling; vasculature development; neuropeptide signaling pathway; regulation of blood pressure; receptor internalization; negative regulation of smooth muscle contraction; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; inflammatory response; negative regulation of bone resorption; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation