Typical Testing Data/Standard Curve (for reference only)
Rabbit RHOB ELISA Kit | RHOB elisa kit
Rabbit RHOB (Ras Homolog Gene Family, Memmber B) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RHOB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RHOB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RHOB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RHOB. You can calculate the concentration of RHOB in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; Tumor suppressor
Chromosomal Location of Human Ortholog: 2p24
Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; nucleus; cytosol; cleavage furrow
Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding
Biological Process: platelet activation; axon guidance; transformed cell apoptosis; apoptosis; metabolic process; positive regulation of apoptosis; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of angiogenesis; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; cell adhesion; blood coagulation
Research Articles on RHOB
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Product Notes
The Rabbit RHOB rhob (Catalog #AAA2511344) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511344 ELISA Kit recognizes Rabbit RHOB. It is sometimes possible for the material contained within the vial of "RHOB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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