Typical Testing Data/Standard Curve (for reference only)
Sheep MYH10 ELISA Kit | MYH10 elisa kit
Sheep MYH10 (Myosin Heavy Chain 10, Non Muscle) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MYH10. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MYH10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MYH10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MYH10. You can calculate the concentration of MYH10 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
MYH10: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECM29. Interacts with KIF26B. Interacts with LARP6. Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 17p13
Cellular Component: mitochondrion; myosin II complex; dendritic spine; actomyosin; cell cortex; actin cytoskeleton; growth cone; cell soma; axon; lamellipodium; cytoplasm; plasma membrane; stress fiber; spindle; midbody; nucleus; neuromuscular junction; myosin complex; cleavage furrow
Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; protein binding; ADP binding; actin-dependent ATPase activity; actin binding; ATP binding
Biological Process: adult heart development; axon guidance; exocytosis; actin filament-based movement; metabolic process; in utero embryonic development; fourth ventricle development; ventricular cardiac muscle cell development; actomyosin structure organization and biogenesis; neuron migration; cerebellar Purkinje cell layer development; cardiac myofibril assembly; third ventricle development; substrate-bound cell migration, cell extension; regulation of cell shape; cell proliferation; cytokinesis after mitosis; plasma membrane repair; retina development in camera-type eye; ephrin receptor signaling pathway; neuromuscular process controlling balance; cell adhesion; nuclear migration; lateral ventricle development
Research Articles on MYH10
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Product Notes
The Sheep MYH10 myh10 (Catalog #AAA2510309) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510309 ELISA Kit recognizes Sheep MYH10. It is sometimes possible for the material contained within the vial of "MYH10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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