Chicken SA ELISA Kit | SA elisa kit
Chicken SA (Sialic Acid) ELISA Kit
Gene Names
ACSM3; SA; SAH
Reactivity
Chicken
Synonyms
SA; Chicken SA (Sialic Acid) ELISA Kit; SA elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken SA. No significant cross-reactivity or interference between Chicken SA and analogues was observed.
Sequence Length
578
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
Related Product Information for SA elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken SA concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SA. During the reaction, SA in the sample or standard competes with a fixed amount of SA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SA. During the reaction, SA in the sample or standard competes with a fixed amount of SA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,485 Da
NCBI Official Full Name
SA
NCBI Official Synonym Full Names
acyl-CoA synthetase medium-chain family member 3
NCBI Official Symbol
ACSM3
NCBI Official Synonym Symbols
SA; SAH
NCBI Protein Information
acyl-coenzyme A synthetase ACSM3, mitochondrial; SA (rat hypertension-associated) homolog; SA hypertension-associated homolog; butyrate--CoA ligase 3; butyryl-coenzyme A synthetase 3; middle-chain acyl-CoA synthetase 3; protein SA homolog
UniProt Protein Name
Acyl-coenzyme A synthetase ACSM3, mitochondrial
Protein Family
UniProt Gene Name
ACSM3
UniProt Synonym Gene Names
SAH
UniProt Entry Name
ACSM3_HUMAN
Uniprot Description
ACSM3: Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4- unsaturated acids (in vitro). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Carbohydrate Metabolism - butanoate; Ligase; EC 6.2.1.2
Chromosomal Location of Human Ortholog: 16p13.11
Cellular Component: mitochondrial matrix
Molecular Function: butyrate-CoA ligase activity; fatty-acid ligase activity; metal ion binding; ATP binding
Biological Process: cholesterol homeostasis; regulation of blood pressure; fatty acid biosynthetic process
Research Articles on SA
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Product Notes
The Chicken SA acsm3 (Catalog #AAA2508865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508865 ELISA Kit recognizes Chicken SA. It is sometimes possible for the material contained within the vial of "SA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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