Chicken CAP1 ELISA Kit | CAP1 elisa kit

Chicken CAP1 (Adenylate Cyclase Associated Protein 1) ELISA Kit

Cat. Num. AAA2506022

• Gene Names: Park7; Dj1; CAP1; DJ-1; SP22
• Reactivity: Chicken
• Synonyms: CAP1; Chicken CAP1 (Adenylate Cyclase Associated Protein 1) ELISA Kit; CAP1 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken CAP1. No significant cross-reactivity or interference between Chicken CAP1 and analogues was observed.
• Sequence Length: 189

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100pg/mL
• Sensitivity: Min: 0.938pg/mL; Max: 100pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAP1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CAP1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CAP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CAP1. You can calculate the concentration of CAP1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 3250916
• NCBI GeneID: 117287
• UniProt Accession #: O88767
• Molecular Weight: 19,974 Da
• NCBI Official Full Name: CAP1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; CAP1; DJ-1; SP22
• NCBI Protein Information: protein DJ-1; Parkinson disease (autosomal recessive, early onset) 7; contraception-associated protein 1; fertility protein SP22; parkinson disease protein 7 homolog
• UniProt Protein Name: Protein DJ-1
• Protein Family: CAP1-binding-protein
• UniProt Gene Name: Park7
• UniProt Synonym Gene Names: Cap1; Protein CAP1
• UniProt Entry Name: PARK7_RAT

NCBI Description

displays a specific localization pattern to the anterior-ventral surface of the equatorial segment; may play a role in sperm-egg interactions and fertilization [RGD, Feb 2006]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Tumor suppressor; Nuclear receptor co-regulator; EC 3.4.-.-; Transcription regulation

Cellular Component: PML body; mitochondrion; endoplasmic reticulum; axon; cytoplasm; plasma membrane; nucleus; cytosol; mitochondrial respiratory chain complex I; chromatin; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; peptidase activity; mRNA binding; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding

Biological Process: synaptic transmission, dopaminergic; negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; response to drug; mitochondrion organization and biogenesis; protein stabilization; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; membrane depolarization; fertilization; response to hydrogen peroxide; detoxification of mercury ion; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; negative regulation of protein kinase activity; autophagy; negative regulation of protein ubiquitination; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of apoptosis