Typical Testing Data/Standard Curve (for reference only)
Sheep ELA2B ELISA Kit | ELA2B elisa kit
Sheep ELA2B (Elastase 2B) ELISA Kit
Gene Names
CELA2B; ELA2B
Reactivity
Sheep
Synonyms
ELA2B; Sheep ELA2B (Elastase 2B) ELISA Kit; ELA2B elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and recombinant Sheep ELA2B. No significant cross-reactivity or interference between Sheep ELA2B and analogues was observed.
Sequence Length
269
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ELA2B elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep ELA2B concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELA2B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELA2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELA2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELA2B. You can calculate the concentration of ELA2B in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ELA2B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ELA2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ELA2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ELA2B. You can calculate the concentration of ELA2B in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,810 Da
NCBI Official Full Name
elastase 2B
NCBI Official Synonym Full Names
chymotrypsin-like elastase family, member 2B
NCBI Official Symbol
CELA2B
NCBI Official Synonym Symbols
ELA2B
NCBI Protein Information
chymotrypsin-like elastase family member 2B; RP11-265F14.2; elastase 2B; elastase-2B; pancreatic elastase IIB (ELA2B)
UniProt Protein Name
Chymotrypsin-like elastase family member 2B
UniProt Gene Name
CELA2B
UniProt Synonym Gene Names
ELA2B
UniProt Entry Name
CEL2B_HUMAN
NCBI Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2B is secreted from the pancreas as a zymogen. In other species, elastase 2B has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. [provided by RefSeq, Jul 2008]
Uniprot Description
CELA2B: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.71; Protease
Chromosomal Location of Human Ortholog: 1p36.21
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on ELA2B
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Product Notes
The Sheep ELA2B cela2b (Catalog #AAA2505949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505949 ELISA Kit recognizes Sheep ELA2B. It is sometimes possible for the material contained within the vial of "ELA2B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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