Typical Testing Data/Standard Curve (for reference only)
Rat TRH ELISA Kit | TRH elisa kit
Rat TRH (Thyrotropin-Releasing Hormone) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat TRH. During the reaction, Rat TRH in the sample or standard competes with a fixed amount of Rat TRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat TRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Rat TRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]
Uniprot Description
TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q13.3-q21
Cellular Component: plasma membrane; extracellular region; nucleus; secretory granule
Molecular Function: neuropeptide hormone activity; thyrotropin-releasing hormone activity
Biological Process: negative regulation of glutamate secretion; hormone-mediated signaling; eating behavior; positive regulation of insulin secretion; histamine metabolic process; signal transduction; adult walking behavior; response to ethanol; cell-cell signaling; response to hypoxia; response to glucose stimulus; response to cold; positive regulation of gamma-aminobutyric acid secretion; response to corticosterone stimulus
Disease: Thyrotropin-releasing Hormone Deficiency
Research Articles on TRH
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Product Notes
The Rat TRH trh (Catalog #AAA2504273) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504273 ELISA Kit recognizes Rat TRH. It is sometimes possible for the material contained within the vial of "TRH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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