Porcine REG3gamma ELISA Kit | REG3gamma elisa kit
Porcine REG3gamma (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit
Gene Names
REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
Reactivity
Porcine
Synonyms
REG3gamma; Porcine REG3gamma (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit; REG3gamma elisa kit
Reactivity
Porcine
Specificity
This kit recognizes Porcine PIICP in samples. No Significant cross-reactivity or interference between Porcine PIICP and analogues was observed.
Sequence Length
129
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine PIICP were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine PIICP were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Related Product Information for REG3gamma elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine PIICP concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PIICP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PIICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PIICP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine PIICP. You can calculate the concentration of Porcine PIICP in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PIICP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PIICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PIICP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine PIICP. You can calculate the concentration of Porcine PIICP in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,409 Da
NCBI Official Full Name
regenerating islet-derived protein 3-gamma isoform 2 preproprotein
NCBI Official Synonym Full Names
regenerating islet-derived 3 gamma
NCBI Official Symbol
REG3G
NCBI Official Synonym Symbols
PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-gamma; REG-3-gamma; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; reg III-gamma; regenerating gene III; regenerating islet-derived protein III-gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
UniProt Gene Name
REG3G
UniProt Synonym Gene Names
PAP1B; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma
UniProt Entry Name
REG3G_HUMAN
NCBI Description
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Nov 2014]
Uniprot Description
REG3G: Might be a stress protein involved in the control of bacterial proliferation.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: cytoplasm; extracellular region
Molecular Function: carbohydrate binding
Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response
Research Articles on REG3gamma
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Product Notes
The Porcine REG3gamma reg3g (Catalog #AAA2503802) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503802 ELISA Kit recognizes Porcine REG3gamma. It is sometimes possible for the material contained within the vial of "REG3gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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