Typical Testing Data/Standard Curve (for reference only)
Human REG3alpha ELISA Kit | REG3alpha elisa kit
Human REG3alpha (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit
Gene Names
Reg3a; AV063448
Reactivity
Human
Synonyms
REG3alpha; Human REG3alpha (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit; REG3alpha elisa kit
Reactivity
Human
Specificity
This kit recognizes Human RBP4 in samples. No significant cross-reactivity or interference between Human RBP4 and analogues was observed.
Sequence Length
175
Samples
Serum, Plasma, Urine. Please Consult Technical Support For The Applicability If Other Biological Fluids Need To Be Tested
Assay Type
Quantitative Sandwich
Detection Range
3.13-100ng/mL
Sensitivity
1.98ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RBP4 were tested 20 times on one plate, respectively
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RBP4 were tested on 3 different plates,20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for REG3alpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RBP4 concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RBP4. Samples (or Standards) and biotinylated detection antibody specific for Human RBP4 are added to the micro ELISA plate wells. Human RBP4 would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human RBP4. You can calculate the concentration of Human RBP4 in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Retinol binding protein 4(RBP4), previously called retinol binding protein(RBP), is a transporter protein. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood [1]. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A, blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells.RBP4 seems to be correlated with cardio metabolic markers in inflammatory chronic diseases, including obesity, type 2 diabetes, metabolic syndrome, and cardiovascular diseases (CVDs) [2].
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RBP4. Samples (or Standards) and biotinylated detection antibody specific for Human RBP4 are added to the micro ELISA plate wells. Human RBP4 would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human RBP4. You can calculate the concentration of Human RBP4 in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Retinol binding protein 4(RBP4), previously called retinol binding protein(RBP), is a transporter protein. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood [1]. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A, blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells.RBP4 seems to be correlated with cardio metabolic markers in inflammatory chronic diseases, including obesity, type 2 diabetes, metabolic syndrome, and cardiovascular diseases (CVDs) [2].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,539 Da
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating islet-derived 3 alpha
NCBI Official Symbol
Reg3a
NCBI Official Synonym Symbols
AV063448
NCBI Protein Information
regenerating islet-derived protein 3-alpha; REG-3-alpha; RegIII (alpha); generating islet-derived, mouse homolog 3 alpha; islet of Langerhans regenerating protein 3; lithostathine 3; pancreatitis-associated protein 2; reg III-alpha; regenerating islet-derived protein III-alpha
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
UniProt Gene Name
Reg3a
UniProt Synonym Gene Names
Pap2; REG-3-alpha; Reg III-alpha
UniProt Entry Name
REG3A_MOUSE
Uniprot Description
REG3A: Might be a stress protein involved in the control of bacterial proliferation.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region
Molecular Function: carbohydrate binding
Biological Process: negative regulation of keratinocyte differentiation; acute-phase response; inflammatory response
Research Articles on REG3alpha
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Product Notes
The Human REG3alpha reg3a (Catalog #AAA2502929) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2502929 ELISA Kit recognizes Human REG3alpha. It is sometimes possible for the material contained within the vial of "REG3alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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