Typical Testing Data/Standard Curve (for reference only)
Chicken Grb2 ELISA Kit | Grb2 elisa kit
Chicken Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit
Gene Names
GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
Reactivity
Chicken
Synonyms
Grb2; Chicken Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit; Grb2 elisa kit
Reactivity
Chicken
Specificity
This kit recognizes natural and recombinant Chicken Grb2. No significant cross-reactivity or interference between Chicken Grb2 and analogues was observed.
Sequence Length
217
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Grb2 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken Grb2 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Grb2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Grb2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Grb2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Grb2. You can calculate the concentration of Grb2 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Grb2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Grb2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Grb2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Grb2. You can calculate the concentration of Grb2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,557 Da
NCBI Official Full Name
growth factor receptor-bound protein 2 isoform 1
NCBI Official Synonym Full Names
growth factor receptor-bound protein 2
NCBI Official Symbol
GRB2
NCBI Official Synonym Symbols
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
NCBI Protein Information
growth factor receptor-bound protein 2; HT027; SH2/SH3 adapter GRB2; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3; protein Ash
UniProt Protein Name
Growth factor receptor-bound protein 2
Protein Family
UniProt Gene Name
GRB2
UniProt Synonym Gene Names
ASH
UniProt Entry Name
GRB2_HUMAN
NCBI Description
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Research Articles on Grb2
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Product Notes
The Chicken Grb2 grb2 (Catalog #AAA2502888) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2502888 ELISA Kit recognizes Chicken Grb2. It is sometimes possible for the material contained within the vial of "Grb2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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