Rabbit BCAN ELISA Kit | BCAN elisa kit

Rabbit BCAN (Brevican) ELISA Kit

Cat. Num. AAA2502175

• Gene Names: BCAN; BEHAB; CSPG7
• Reactivity: Rabbit
• Synonyms: BCAN; Rabbit BCAN (Brevican) ELISA Kit; BCAN elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit BCAN. No significant cross-reactivity or interference between Rabbit BCAN and analogues was observed.
• Sequence Length: 911

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCAN elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit BCAN concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BCAN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BCAN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BCAN. You can calculate the concentration of BCAN in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38372935
• NCBI GeneID: 63827
• NCBI Accession #: NP_068767.3
• NCBI GenBank Nucleotide #: NM_021948.4
• UniProt Accession #: Q96GW7; Q5SZ10; Q5T3I5; Q8TBB9; Q9HBK1; Q9HBK4; D3DVC2
• Molecular Weight: 71,671 Da
• NCBI Official Full Name: brevican core protein isoform 1
• NCBI Official Synonym Full Names: brevican
• NCBI Official Symbol: BCAN
• NCBI Official Synonym Symbols: BEHAB; CSPG7
• NCBI Protein Information: brevican core protein; brain-enriched hyaluronan-binding protein; chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB
• UniProt Protein Name: Brevican core protein
• Protein Family: Brevican core protein
• UniProt Gene Name: BCAN
• UniProt Synonym Gene Names: BEHAB; CSPG7; BEHAB
• UniProt Entry Name: PGCB_HUMAN

NCBI Description

This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

BCAN: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Belongs to the aggrecan/versican proteoglycan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: chondroitin sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; hippocampus development; chondroitin sulfate catabolic process; pathogenesis; cell adhesion; skeletal development; dermatan sulfate biosynthetic process