Porcine SRP9 ELISA Kit | SRP9 elisa kit

Porcine SRP9 (Signal Recognition Particle 9kDa) ELISA Kit

Cat. Num. AAA2500944

• Gene Names: SRP9; ALURBP
• Reactivity: Porcine
• Synonyms: SRP9; Porcine SRP9 (Signal Recognition Particle 9kDa) ELISA Kit; SRP9 elisa kit

• Reactivity: Porcine
• Specificity: This kit recognizes natural and recombinant Porcine SRP9. No significant cross-reactivity or interference between Porcine SRP9 and analogues was observed.
• Sequence Length: 86

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for SRP9 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine SRP9 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SRP9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SRP9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SRP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SRP9. You can calculate the concentration of SRP9 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507217
• NCBI GeneID: 6726
• NCBI Accession #: NP_003124.1
• NCBI GenBank Nucleotide #: NM_003133.5
• UniProt Accession #: P49458; Q6NVX0; Q8WTW0; A8K0N0
• Molecular Weight: 9,124 Da
• NCBI Official Full Name: signal recognition particle 9 kDa protein isoform 2
• NCBI Official Synonym Full Names: signal recognition particle 9kDa
• NCBI Official Symbol: SRP9
• NCBI Official Synonym Symbols: ALURBP
• NCBI Protein Information: signal recognition particle 9 kDa protein; signal recognition particle 9kD
• UniProt Protein Name: Signal recognition particle 9 kDa protein
• Protein Family: Signal recognition particle 9 kDa protein
• UniProt Gene Name: SRP9
• UniProt Synonym Gene Names: SRP9
• UniProt Entry Name: SRP09_HUMAN

Uniprot Description

SRP9: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding. Belongs to the SRP9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: signal recognition particle receptor complex; signal recognition particle, endoplasmic reticulum targeting; cytosol

Molecular Function: signal recognition particle binding; RNA binding; 7S RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; negative regulation of translational elongation; gene expression