Mouse anti-Human AQP1 / Aquaporin 1 Monoclonal Antibody | anti-AQP1 antibody

Anti-AQP1 / Aquaporin 1 Antibody (clone 1F3) IHC-plus

Cat. Num. AAA249402

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: AQP1 / Aquaporin 1; Monoclonal Antibody; Anti-AQP1 / Aquaporin 1 Antibody (clone 1F3) IHC-plus; Mouse Monoclonal [clone 1F3] (IgG1) to Human AQP1 / Aquaporin 1; Human AQP1 / Aquaporin 1; Aquaporin-1; AQP-1; AQP-CHIP; Aquaporin 1; Aquaporin-CHIP; CHIP28; Colton blood group; Urine water channel; CO; anti-AQP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1F3
• Specificity: Human AQP1 / Aquaporin 1
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.7 mg/ml (varies by lot)
• Sequence Length: 269

• Applicable Applications for anti-AQP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IHC-P (5 ug/ml); WB (1:2000)
• Immunogen: AQP1 / Aquaporin 1 antibody was raised against full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-AQP1 / Aquaporin 1 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-AQP1 / Aquaporin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AQP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AQP1.)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AQP1 antibody, and then analyzed by flow cytometry.)

Related Product Information for anti-AQP1 antibody

AQP1 Antibody, Aquaporin-1 Antibody, AQP-1 Antibody, AQP-CHIP Antibody, Aquaporin 1 Antibody, Aquaporin-CHIP Antibody, CHIP28 Antibody, Colton blood group Antibody, Urine water channel Antibody, CO Antibody Description: Aquaporin-1 (AQP1), also known as Channel-forming integral membrane protein (CHIP), is the first member of the aquaporin water channel family. It plays a role in the transmembrane water transport, especially in kidneys, red blood cells, central nervous system and some epithelial surfaces. Its activity can be blocked by mercurial compounds. AQP1 is associated with so-called Colton blood group antigens.

NCBI and Uniprot Product Information

• NCBI GI #: 37694062
• NCBI GeneID: 358
• NCBI Accession #: NP_932766.1
• NCBI GenBank Nucleotide #: NM_198098.2
• UniProt Accession #: P29972; Q8TBI5; Q8TDC1; B5BU39; E7EM69; E9PC21; F5GY19
• Molecular Weight: 16,677 Da
• NCBI Official Full Name: aquaporin-1 isoform 1
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Membrane protein, integral; Transporter, aquaporin family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: apical part of cell; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; brush border; brush border membrane; cytoplasm; integral to plasma membrane; nerve terminal; nuclear membrane; nucleus; plasma membrane; sarcolemma

Molecular Function: ammonium transmembrane transporter activity; ephrin receptor binding; glycerol channel activity; glycerol transmembrane transporter activity; intracellular cGMP activated cation channel activity; nitric oxide transporter activity; potassium channel activity; potassium ion transmembrane transporter activity; protein binding; transmembrane transporter activity; water channel activity; water transporter activity

Biological Process: adrenocorticotropin hormone secretion; ammonium transport; bicarbonate transport; camera-type eye morphogenesis; carbon dioxide transport; cell volume homeostasis; cellular homeostasis; cellular response to stress; cerebrospinal fluid secretion; cGMP biosynthetic process; establishment and/or maintenance of actin cytoskeleton polarity; glomerular filtration; glycerol transport; hyperosmotic salinity response; lateral ventricle development; lipid digestion; multicellular organismal water homeostasis; negative regulation of apoptosis; negative regulation of caspase activity; nitric oxide transport; odontogenesis; pancreatic juice secretion; positive regulation of angiogenesis; positive regulation of fibroblast proliferation; positive regulation of saliva secretion; potassium ion transport; renal water homeostasis; renal water transport; response to drug; response to estrogen stimulus; secretory granule organization and biogenesis; sensory perception of pain; transmembrane transport; water transport; wound healing

Disease: Blood Group--colton