Immunohistochemistry (IHC)
(Anti-PGAM2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Mouse PGAM2 Monoclonal Antibody | anti-PGAM2 antibody
Mouse Monoclonal [clone 4E9] (IgG2a) to Human PGAM2
Immunohistochemistry (IHC)
(Anti-PGAM2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-PGAM2 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunofluorescence (IF)
(Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.)
Immunofluorescence (IF)
(Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.)
Immunofluorescence (IF)
(Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.)
Immunofluorescence (IF)
(Anti-PGAM2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody, and then analyzed by flow cytometry.)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.)
Western Blot (WB)
(Western blot of extracts (35 ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.)
NCBI and Uniprot Product Information
NCBI Description
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]
Uniprot Description
PGAM2: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
Protein type: EC 5.4.2.11; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.4
Chromosomal Location of Human Ortholog: 7p13-p12
Cellular Component: nucleus; cytosol
Molecular Function: phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; cofactor binding; 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity
Biological Process: response to mercury ion; striated muscle contraction; glycolysis; dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; spermatogenesis; gluconeogenesis
Disease: Glycogen Storage Disease X
Research Articles on PGAM2
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Product Notes
The PGAM2 pgam2 (Catalog #AAA249017) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4E9] (IgG2a) to Human PGAM2 reacts with Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PGAM2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flo (1:100), IF (1:50 - 1:100), IHC-P (1:150, 10 ug/ml), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the PGAM2 pgam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAM2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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