Immunohistochemistry (IHC)
(Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Rabbit anti-Human, Mouse SPTAN1 / Alpha Fodrin Polyclonal Antibody | anti-SPTAN1 antibody
Rabbit Polyclonal to Human SPTAN1 / Alpha Fodrin
Usage: The serum can be diluted to 1:500-1:1000 for immunofluorescence staining and 1:5000-1:10000 for western blotting. On western blots look for a major band at 240kDa, depending on the species.
Immunohistochemistry (IHC)
(Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunocytochemistry (ICC)
(Mixed neuron-glial cultures stained with rabbit antibody to alpha-II spectrin (green) and mouse polyclonal antibody to Nestin (red). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.)
Immunocytochemistry (ICC)
(Mixed neuron-glial cultures stained with rabbit antibody to alpha-II spectrin (green) and mouse polyclonal antibody to Nestin (red). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.)
Western Blot (WB)
(Western blots of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240 kDa represents the intact alpha-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha-II spectrin.)
Western Blot (WB)
(Western blots of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240 kDa represents the intact alpha-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha-II spectrin.)
NCBI and Uniprot Product Information
NCBI Description
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Uniprot Description
SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: spectrin; microtubule cytoskeleton; membrane; intracellular membrane-bound organelle; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane
Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding
Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis
Disease: Epileptic Encephalopathy, Early Infantile, 5
Research Articles on SPTAN1
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Product Notes
The SPTAN1 sptan1 (Catalog #AAA248102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human SPTAN1 / Alpha Fodrin reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPTAN1 / Alpha Fodrin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). ICC (1:500 - 1:1000), IF (1:500 - 1:1000), IHC-P (1:100), WB (1:5000 - 1:10000) Usage: The serum can be diluted to 1:500-1:1000 for immunofluorescence staining and 1:5000-1:10000 for western blotting. On western blots look for a major band at 240kDa, depending on the species. Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTAN1 / Alpha Fodrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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