Rabbit HNRNPD / AUF1 Polyclonal Antibody | anti-HNRNPD antibody

Rabbit Polyclonal (IgG) to Human HNRNPD / AUF1

Cat. Num. AAA247514

• Gene Names: HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: HNRNPD / AUF1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human HNRNPD / AUF1; Anti-HNRNPD / AUF1 Antibody (aa49-98) IHC-plus; HNRNPD; AUF1A; AUF1; HNRPD; HnRNP D0; p37; HnRNPD0; Human HNRNPD; anti-HNRNPD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: hnRPD Antibody detects endogenous levels of total hnRPD protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 355

• Applicable Applications for anti-HNRNPD antibody: IHC, IHC-Paraffin, Immunofluorescence, Western Blot, Peptide Enzyme-Linked Immunosorbent Assay
• Application Notes: IHC; IHC-Paraffin; Immunofluorescence(1:100-1:500); Western Blot (1:500-1:1000); Peptide Enzyme- Linked Immunosorbent Assay (1:20000); ; Optimal dilution to be determined by the researcher).
• Target Species: Human
• Immunogen Species: HNRNPD/AUF1 antibody was raised against Human
• Immunogen: HNRNPD/AUF1 antibody was raised against synthetic peptide from human hnRPD (aa49-98)
• Epitope: aa49-98
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-HNRNPD / AUF1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunohistochemistry (IHC)

(Anti-HNRNPD / AUF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using hnRPD (Ab-83) Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293/Jurkat/3T3/COLO205 cells, using hnRPD (Ab-83) Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 14110420
• NCBI GeneID: 3184
• NCBI Accession #: NP_112738.1
• NCBI GenBank Nucleotide #: NM_031370.2
• UniProt Accession #: Q14103; P07029; Q01858; Q14100; Q14101; Q14102; Q4W5A1; Q9UCE8; Q9UCE9; A8K9J2
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein D0 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
• NCBI Official Symbol: HNRNPD
• NCBI Official Synonym Symbols: P37; AUF1; AUF1A; HNRPD; hnRNPD0
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; ARE-binding protein AUFI, type A
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein D0
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPD
• UniProt Synonym Gene Names: AUF1; HNRPD; hnRNP D0
• UniProt Entry Name: HNRPD_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP D0: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). Implicated in the regulation of mRNA stability. Has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. Four alternatively spliced transcript variants have been described.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: nucleoplasm; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; positive regulation of translation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; positive regulation of transcription, DNA-dependent; RNA catabolic process; gene expression; regulation of mRNA stability; regulation of circadian rhythm