Rabbit AGER / RAGE Polyclonal Antibody | anti-AGER antibody

Rabbit Polyclonal to Human AGER / RAGE

Cat. Num. AAA247493

• Gene Names: AGER; RAGE
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: AGER / RAGE; Polyclonal Antibody; Rabbit Polyclonal to Human AGER / RAGE; Anti-AGER / RAGE Antibody (aa350-400) IHC-plus; RAGE isoform NtRAGE-delta; RAGE isoform sRAGE-delta; Human AGER; RAGE; anti-AGER antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human RAGE
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 404

• Applicable Applications for anti-AGER antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
• Target Species: Human
• Immunogen Description: Amino acids 350-400 of human RAGE.
• Immunogen Type: Synthetic peptide
• Immunogen: AGER / RAGE antibody was raised against amino acids 350-400 of human RAGE.
• Antigen Modification: aa350-400
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-AGER / RAGE antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Western Blot (WB)

(Western blot of RAGE in 1) human lung tissue lysate, mouse lung tissue lysate in the 2) absence and 3) presence of immunizing peptide, and 4) rat lung tissue lysate using at 3, 0.1 and 0.1 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test. Note: This antibody was raised against an amino acid sequence that is 100% conserved in human, however western blot testing shows that it does not detected the expected ~45 kD band in human lung lysate.)

Related Product Information for anti-AGER antibody

RAGE/AGER is a member of the immunoglobulin super family of cell surface molecules. It binds multiple ligands and interacts with distinct molecules implicated in homeostasis, development, inflammation, and diseases such as carcinoma, diabetes, renal failure, and Alzheimer's. Cell signaling pathways/molecules known to be activated by ligand bound RAGE include p21 (ras), MAP kinases, NF-kappa-B, and cdc42. Binding of amphoterin by RAGE is linked to outgrowth of cortical neurons and tumor invasion.

NCBI and Uniprot Product Information

• NCBI GI #: 10835203
• NCBI GeneID: 177
• NCBI Accession #: NP_001127.1
• NCBI GenBank Nucleotide #: NM_001136.4
• UniProt Accession #: Q15109; Q15279; Q3L1R4; Q3L1R5; Q3L1R6; Q3L1R7; Q3L1R8; Q3L1S0; A2BFI7; A6NKF0; A7Y2U9; B0V176
• Molecular Weight: 41,098 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 1
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta; receptor for advanced glycation end-products variant 20
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE
• UniProt Entry Name: RAGE_HUMAN

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: cell surface receptor linked signal transduction; response to wounding; innate immune response; inflammatory response; neurite development; activation of NF-kappaB transcription factor; induction of positive chemotaxis