Rabbit UBE1DC1 / UBA5 Polyclonal Antibody | anti-UBE1DC1 antibody

Rabbit Polyclonal (IgG) to Human UBE1DC1 / UBA5

Cat. Num. AAA247097

• Gene Names: UBA5; THIFP1; UBE1DC1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: UBE1DC1 / UBA5; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human UBE1DC1 / UBA5; Anti-UBE1DC1 / UBA5 Antibody (aa281-330) IHC-plus; UBA5; THIFP1; Ubiquitin-activating enzyme 5; UBE1DC1; UFM1-activating enzyme; Human UBE1DC1; anti-UBE1DC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBA5 Antibody detects endogenous levels of total UBA5 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 404

• Applicable Applications for anti-UBE1DC1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human UBA5 (aa281-330).
• Immunogen: UBE1DC1 / UBA5 antibody was raised against synthetic peptide from human UBA5 (aa281-330).
• Antigen Modification: aa281-330
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-UBA5 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HepG2/293 cells, using UBA5 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 13376212
• NCBI GeneID: 79876
• NCBI Accession #: NP_079094.1
• NCBI GenBank Nucleotide #: NM_024818.3
• UniProt Accession #: Q9GZZ9; Q96ST1; A6NJL3; D3DNC8
• Molecular Weight: 38,537 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway