Mouse RPS7 Monoclonal Antibody | anti-RPS7 antibody

Mouse Monoclonal [clone 3G4] (IgG1,k) to Human RPS7

Cat. Num. AAA246655

• Gene Names: RPS7; S7; DBA8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RPS7; Monoclonal Antibody; Mouse Monoclonal [clone 3G4] (IgG1; k) to Human RPS7; Anti-RPS7 Antibody (clone 3G4) IHC-plus; 40S ribosomal protein S7; Ribosomal protein S7; DBA8; Human RPS7; anti-RPS7 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G4
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 194

• Applicable Applications for anti-RPS7 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Raw 264. Western Blot (Cell lysate) - positive control NIH/3T3. Immunofluorescence (20 ug/ml) - po...
• Target Species: Human
• Immunogen Description: RPS7 (NP_001002, 95 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: RPS7 antibody was raised against rPS7 (NP_001002, 95 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-RPS7 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-RPS7 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell (antibody concentration 20 ug/ml).)

Western Blot (WB)

(RPS7 monoclonal antibody clone 3G4 Western blot of RPS7 expression in HeLa.)

Western Blot (WB)

(RPS7 monoclonal antibody clone 3G4. Western blot of RPS7 expression in NIH/3T3.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RPS7 is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506741
• NCBI GeneID: 6201
• NCBI Accession #: NP_001002.1
• NCBI GenBank Nucleotide #: NM_001011.3
• UniProt Accession #: P62081; P23821; P24818; Q57Z92; Q6IPH1
• Molecular Weight: 22,127 Da
• NCBI Official Full Name: 40S ribosomal protein S7
• NCBI Official Synonym Full Names: ribosomal protein S7
• NCBI Official Symbol: RPS7
• NCBI Official Synonym Symbols: S7; DBA8
• NCBI Protein Information: 40S ribosomal protein S7
• UniProt Protein Name: 40S ribosomal protein S7
• Protein Family: Ribosomal protein
• UniProt Gene Name: RPS7
• UniProt Entry Name: RS7_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS7: Required for rRNA maturation. Defects in RPS7 are the cause of Diamond-Blackfan anemia type 8 (DBA8). DBA8 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein S7e family.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: small subunit processome; focal adhesion; membrane; cytoplasm; nucleolus; ribosome; microtubule organizing center; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; viral infectious cycle; translational termination; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; rRNA processing

Disease: Diamond-blackfan Anemia 8