Mouse GOT2 Monoclonal Antibody | anti-GOT2 antibody

Mouse Monoclonal [clone 3E9] (IgG1) to Human GOT2

Cat. Num. AAA245898

• Gene Names: GOT2; KAT4; KATIV; mitAAT
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Ascites
• Synonyms: GOT2; Monoclonal Antibody; Mouse Monoclonal [clone 3E9] (IgG1) to Human GOT2; Anti-GOT2 Antibody (clone 3E9) IHC-plus; FABP-1; MitAAT; Transaminase A; FABPpm; Fatty acid-binding protein; KAT4; KATIV; Kynurenine aminotransferase IV; MAspAT; Human GOT2; anti-GOT2 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3E9
• Specificity: Human GOT2
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide
• Sequence Length: 430

• Applicable Applications for anti-GOT2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:200 - 1:1000), IHC-P (1:200), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of human GOT2 expressed in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: GOT2 antibody was raised against purified recombinant fragment of human GOT2 expressed in E Coli.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-GOT2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-GOT2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Western Blot (WB)

(Western blot of GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.)

Related Product Information for anti-GOT2 antibody

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

NCBI and Uniprot Product Information

• NCBI GI #: 73486658
• NCBI GeneID: 2806
• NCBI Accession #: NP_002071.2
• NCBI GenBank Nucleotide #: NM_002080.3
• UniProt Accession #: P00505; Q53FL3; Q9BWA3; B4DJA6; E7ERW2
• Molecular Weight: 43,030 Da
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 2, mitochondrial
• NCBI Official Symbol: GOT2
• NCBI Official Synonym Symbols: KAT4; KATIV; mitAAT
• NCBI Protein Information: aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate transaminase 2; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; plasma membrane-associated fatty acid-binding protein; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: GOT2
• UniProt Synonym Gene Names: mAspAT; FABP-1; FABPpm
• UniProt Entry Name: AATM_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transferase; EC 2.6.1.7; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.1; Mitochondrial; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; aspartate biosynthetic process; glucose metabolic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process; fatty acid transport