Immunohistochemistry (IHC)
(Anti-MHC Class II RT-1B antibody IHC of rat spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse anti-Mouse, Rat MHC Class II RT-1B Monoclonal Antibody
Mouse Monoclonal [clone OX-6] (IgG1) to Rat MHC Class II RT-1B
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein G Purified
Synonyms
MHC Class II RT-1B; Monoclonal Antibody; Mouse Monoclonal [clone OX-6] (IgG1) to Rat MHC Class II RT-1B; Anti-MHC Class II RT-1B Antibody (clone OX-6) IHC-plus; Rat MHC Class II RT-1B; anti-MHC Class II RT-1B antibody
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-6
Specificity
Recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression. also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region.
Purity/Purification
Protein G Purified
Form/Format
PBS, pH 7.2, 0.09% sodium azide.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-MHC Class II RT-1B antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Flo (1:50 - 1:100), IHC-Fr, IHC-P (10 ug/ml), WB
Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)
Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)
Target Species
Rat
Immunogen Description
Rat thymocyte membrane glycoprotein.
Immunogen Type
Membrane extract
Immunogen
Rat thymocyte membrane glycoprotein.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-MHC Class II RT-1B antibody IHC of rat spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-MHC Class II RT-1B antibody IHC of rat spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
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Product Notes
The MHC Class II RT-1B (Catalog #AAA245890) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone OX-6] (IgG1) to Rat MHC Class II RT-1B reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class II RT-1B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Flow Cytometry (FC/FACS). Flo (1:50 - 1:100), IHC-Fr, IHC-P (10 ug/ml), WB Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Researchers should empirically determine the suitability of the MHC Class II RT-1B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class II RT-1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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