Mouse anti-Human NEUROD1 / Beta2 Monoclonal Antibody | anti-NEUROD1 antibody

Mouse Monoclonal [clone 3D11] (IgG) to Human NEUROD1 / Beta2

Cat. Num. AAA245800

• Gene Names: NEUROD1; BETA2; BHF-1; MODY6; NEUROD; bHLHa3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: NEUROD1 / Beta2; Monoclonal Antibody; Mouse Monoclonal [clone 3D11] (IgG) to Human NEUROD1 / Beta2; Anti-NEUROD1 / Beta2 Antibody (clone 3D11) IHC-plus; NEUROD1; Beta2; BHF-1; NEUROD; Neurogenic differentiation 1; MODY6; NIDDM; BHLHa3; Neuronal differentiation 1; Human NEUROD1; anti-NEUROD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 3D11
• Specificity: Human NeuroD1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 356

• Applicable Applications for anti-NEUROD1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using transfected cell lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: NEUROD1 (AAH09046, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: NEUROD1 / Beta2 antibody was raised against nEUROD1 (AAH09046, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NEUROD1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody clone 3D11.)

ELISA (EIA)

(Detection limit for recombinant GST tagged NEUROD1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 121114306
• NCBI GeneID: 4760
• NCBI Accession #: NP_002491.2
• NCBI GenBank Nucleotide #: NM_002500.4
• UniProt Accession #: Q13562; O00343; Q13340; Q5U095; Q96TH0; Q99455; Q9UEC8; B2R9I8; F1T0E1
• Molecular Weight: 39,920 Da
• NCBI Official Full Name: neurogenic differentiation factor 1
• NCBI Official Synonym Full Names: neuronal differentiation 1
• NCBI Official Symbol: NEUROD1
• NCBI Official Synonym Symbols: BETA2; BHF-1; MODY6; NEUROD; bHLHa3
• NCBI Protein Information: neurogenic differentiation factor 1; beta-cell E-box transactivator 2; class A basic helix-loop-helix protein 3; neurogenic helix-loop-helix protein NEUROD; basic helix-loop-helix transcription factor
• UniProt Protein Name: Neurogenic differentiation factor 1
• UniProt Gene Name: NEUROD1
• UniProt Synonym Gene Names: BHLHA3; NEUROD; NeuroD; NeuroD1; bHLHa3
• UniProt Entry Name: NDF1_HUMAN

NCBI Description

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]

Uniprot Description

NEUROD1: Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulates transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor p21Cip1. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus). Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E- box element. Efficient DNA-binding requires dimerization with another bHLH protein. Interacts with RREB1. Interacts with EP300; the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: response to drug; dentate gyrus development; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; nucleocytoplasmic transport; glucose homeostasis; endocrine pancreas development; anterior/posterior pattern formation; embryonic organ morphogenesis; neurogenesis; insulin secretion; response to glucose stimulus; cerebellum development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of neuron differentiation; regulation of insulin secretion; negative regulation of JAK-STAT cascade; nitric oxide mediated signal transduction; inner ear development

Disease: Maturity-onset Diabetes Of The Young, Type 6; Diabetes Mellitus, Noninsulin-dependent