Mouse anti-Human FGG / Fibrinogen Gamma Monoclonal Antibody | anti-FGG antibody

Mouse Monoclonal [clone 1F2] (IgG1,k) to Human FGG / Fibrinogen Gamma

Cat. Num. AAA245786

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: FGG / Fibrinogen Gamma; Monoclonal Antibody; Mouse Monoclonal [clone 1F2] (IgG1; k) to Human FGG / Fibrinogen Gamma; Anti-FGG / Fibrinogen Gamma Antibody (clone 1F2) IHC-plus; FGG; Fibrinogen gamma-b chain; Fibrinogen gamma chain; Fibrinogen; gamma polypeptide; Human FGG; Fibrinogen Gamma; anti-FGG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1F2
• Specificity: Human Fibrinogen Gamma-b Chain
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.49 mg/ml (varies by lot)
• Sequence Length: 453

• Applicable Applications for anti-FGG antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.
• Target: FGG/Fibrinogen Gamma
• Immunogen Species: FGG/Fibrinogen Gamma antibody was raised against Human.
• Target Species: Human
• Immunogen Description: FGG (AAH07044, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: FGG / Fibrinogen Gamma antibody was raised against fGG (AAH07044, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-FGG / Fibrinogen Gamma Chain antibody IHC of human intravascular plasma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(FGG monoclonal antibody clone 1F2 Western blot of FGG expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged FGG is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 70906439
• NCBI GeneID: 2266
• NCBI Accession #: NP_068656.2
• NCBI GenBank Nucleotide #: NM_021870.2
• UniProt Accession #: P02679; P04469; P04470; Q53Y18; Q96A14; Q96KJ3; Q9UC62; Q9UC63; Q9UCF3; A8K057
• NCBI Official Full Name: fibrinogen gamma chain isoform gamma-B
• NCBI Official Synonym Full Names: fibrinogen gamma chain
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma chain; fibrinogen, gamma polypeptide
• UniProt Protein Name: Fibrinogen gamma chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Entry Name: FIBG_HUMAN

NCBI Description

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FGG: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGG are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region; plasma membrane; cell cortex; external side of plasma membrane

Molecular Function: protein binding, bridging; metal ion binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; platelet degranulation; cellular protein complex assembly; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; response to calcium ion; signal transduction; blood coagulation; positive regulation of exocytosis

Disease: Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital