Mouse anti-Human NDN / Necdin Monoclonal Antibody | anti-NDN antibody

Mouse Monoclonal [clone 1B3] (IgG2a,k) to Human NDN / Necdin

Cat. Num. AAA245716

• Gene Names: NDN; PWCR; HsT16328
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: NDN / Necdin; Monoclonal Antibody; Mouse Monoclonal [clone 1B3] (IgG2a; k) to Human NDN / Necdin; Anti-NDN / Necdin Antibody (clone 1B3) IHC-plus; NDN; Necdin; Necdin homolog (mouse); HsT16328; Necdin (mouse) homolog; PWCR; Human NDN; anti-NDN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1B3
• Specificity: Human Necdin
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.38 mg/ml (varies by lot)
• Sequence Length: 321

• Applicable Applications for anti-NDN antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: NDN (NP_002478, 222 a.a. ~ 322 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: NDN / Necdin antibody was raised against nDN (NP_002478, 222 a.a. ~ 322 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NDN / Necdin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of NDN expression in transfected 293T cell line by NDN monoclonal antibody.)

Western Blot (WB)

(NDN monoclonal antibody Western blot of NDN expression in HL-60.)

ELISA (EIA)

(Detection limit for recombinant GST tagged NDN is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505349
• NCBI GeneID: 4692
• NCBI Accession #: NP_002478.1
• NCBI GenBank Nucleotide #: NM_002487.2
• UniProt Accession #: Q99608; B2R6Z5
• Molecular Weight: 36,086 Da
• NCBI Official Full Name: necdin
• NCBI Official Synonym Full Names: necdin, melanoma antigen (MAGE) family member
• NCBI Official Symbol: NDN
• NCBI Official Synonym Symbols: PWCR; HsT16328
• NCBI Protein Information: necdin; necdin homolog; Prader-Willi syndrome chromosome region
• UniProt Protein Name: Necdin
• Protein Family: Necdin
• UniProt Gene Name: NDN
• UniProt Entry Name: NECD_HUMAN

NCBI Description

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008]

Uniprot Description

NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 15q11.2-q12

Cellular Component: centrosome; cell projection; perikaryon; cytosol; nucleus

Molecular Function: gamma-tubulin binding; DNA binding

Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; axon extension; neuron migration; axonal fasciculation; multicellular organismal homeostasis; glial cell migration; sensory perception of pain; post-embryonic development; respiratory system process; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; regulation of growth

Disease: Prader-willi Syndrome