Mouse anti-Human YY1 Monoclonal Antibody | anti-YY1 antibody

Mouse Monoclonal [clone 4A5] (IgG1,k) to Human YY1

Cat. Num. AAA245662

• Gene Names: YY1; DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: YY1; Monoclonal Antibody; Mouse Monoclonal [clone 4A5] (IgG1; k) to Human YY1; Anti-YY1 Antibody (clone 4A5) IHC-plus; Delta transcription factor; INO80S; NF-E1; INO80 complex subunit S; YY1 transcription factor; YIN-YANG-1; YY-1; Yin and yang 1; UCRBP; Yin and Yang 1 protein; Human YY1; anti-YY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4A5
• Specificity: Human YY1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 414

• Applicable Applications for anti-YY1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: YY1 (NP_003394, 221 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: YY1 antibody was raised against yY1 (NP_003394, 221 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-YY1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(YY1 monoclonal antibody clone 4A5 Western blot of YY1 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged YY1 is approximately 0.03 ng/ml as a capture antibody.)

Related Product Information for anti-YY1 antibody

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

NCBI and Uniprot Product Information

• NCBI GI #: 4507955
• NCBI GeneID: 7528
• NCBI Accession #: NP_003394.1
• NCBI GenBank Nucleotide #: NM_003403.4
• UniProt Accession #: P25490; Q14935
• Molecular Weight: 44,713 Da
• NCBI Official Full Name: transcriptional repressor protein YY1
• NCBI Official Synonym Full Names: YY1 transcription factor
• NCBI Official Symbol: YY1
• NCBI Official Synonym Symbols: DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• NCBI Protein Information: transcriptional repressor protein YY1; YY-1; Yin and Yang 1 protein; INO80 complex subunit S; delta transcription factor
• UniProt Protein Name: Transcriptional repressor protein YY1
• Protein Family: YY1-associated factor
• UniProt Gene Name: YY1
• UniProt Synonym Gene Names: INO80S; YY-1
• UniProt Entry Name: TYY1_HUMAN

NCBI Description

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]

Uniprot Description

YY1: a polycomb-group (Pc-G) protein essential for early mammalian development and the regulation of X-inactivation and certain imprinted genes. Required for the transactivation of TSIX by CTCF. The complex of CTCF-YY1-TSIX is a critical regulator of the X chromosome binary switch. Can function as an activator or a repressor depending on the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.

Protein type: C2H2-type zinc finger protein; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 14q

Cellular Component: transcription factor complex; nuclear matrix; PcG protein complex; nucleus

Molecular Function: protein binding; DNA binding; four-way junction DNA binding; zinc ion binding; RNA binding; transcription coactivator activity; SMAD binding; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; anterior/posterior pattern formation; RNA localization; camera-type eye morphogenesis; transcription, DNA-dependent; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; response to DNA damage stimulus; chromosome organization and biogenesis; response to UV-C; double-strand break repair via homologous recombination