5'-Nucleotidase Recombinant Protein | NT5C2 recombinant protein

Recombinant 5'-Nucleotidase, Cytosolic II (NT5C2)

Cat. Num. AAA2034578

• Gene Names: Nt5e; Nt5; CD73
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: 5'-Nucleotidase; Recombinant 5'-Nucleotidase; Cytosolic II (NT5C2); Purine 5' Nucleotidase; 5'-Nucleotidase(Purine); Cytosolic Type B; Cytosolic purine 5'-nucleotidase; NT5C2 recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Human
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 576

• Applicable Applications for NT5C2 recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Met1~Gly212
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Met1~Gly212 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 148747417
• NCBI GeneID: 58813
• NCBI Accession #: NP_067587.2
• NCBI GenBank Nucleotide #: NM_021576.2
• Molecular Weight: Predicted: 28.5kDa
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5' nucleotidase, ecto
• NCBI Official Symbol: Nt5e
• NCBI Official Synonym Symbols: Nt5; CD73
• NCBI Protein Information: 5'-nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• Protein Family: 5'-nucleotidase
• UniProt Gene Name: Nt5e
• UniProt Synonym Gene Names: Nt5; Nte; 5'-NT
• UniProt Entry Name: 5NTD_RAT

NCBI Description

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.3.5; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - pyrimidine

Cellular Component: cell surface; cytoplasm; integral to membrane; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding

Biological Process: adenosine biosynthetic process; adenosine metabolic process; AMP catabolic process; brain development; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; response to aluminum ion