Mouse anti-Human SELPLG / PSGL-1 / CD162 Monoclonal Antibody | anti-SELPLG antibody

Mouse Monoclonal [clone TC2] (IgG1) to Human SELPLG / PSGL-1 / CD162

Cat. Num. AAA245632

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A Purified
• Synonyms: SELPLG / PSGL-1 / CD162; Monoclonal Antibody; Mouse Monoclonal [clone TC2] (IgG1) to Human SELPLG / PSGL-1 / CD162; Anti-SELPLG / PSGL-1 / CD162 Antibody (clone TC2) IHC-plus; SELPLG; CD162 antigen; CLA; CD162; PSGL1; PSGL-1; Selectin P ligand; Human SELPLG; anti-SELPLG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: TC2
• Specificity: The antibody TC2 reacts with CD162, a 220 kD type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 15 mM sodium azide, pH 7.4
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 402

• Applicable Applications for anti-SELPLG antibody: Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1.5 - 2.5 ug/ml), IHC-P (10 ug/ml); Usage: Flow Cytometry. Recommended dilution:1.5 - 2.5 ug/ml. Positive control:human peripheral blood.
• Target Species: Human
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SELPLG / CD162 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-SELPLG antibody

SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.

NCBI and Uniprot Product Information

• NCBI GI #: 4506879
• NCBI GeneID: 6404
• NCBI Accession #: NP_002997.1
• NCBI GenBank Nucleotide #: NM_003006.3
• UniProt Accession #: Q14242; Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6
• Molecular Weight: 44,648 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: leukocyte tethering or rolling; cell adhesion; blood coagulation; leukocyte migration; leukocyte adhesive activation