Mouse anti-Human STAT5B / STAT Monoclonal Antibody | anti-STAT5B antibody

Mouse Monoclonal [clone 5D6] (IgG1,k) to Human STAT5B / STAT

Cat. Num. AAA245576

• Gene Names: STAT5B; STAT5
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: STAT5B / STAT; Monoclonal Antibody; Mouse Monoclonal [clone 5D6] (IgG1; k) to Human STAT5B / STAT; Anti-STAT5B / STAT Antibody (clone 5D6) IHC-plus; STAT5B; Transcription factor STAT5B; STAT5; Human STAT5B; STAT; anti-STAT5B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5D6
• Specificity: Human STAT5b
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4
• Concentration: 0.45 mg/ml (varies by lot)
• Sequence Length: 787

• Applicable Applications for anti-STAT5B antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (25 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target: STAT5B
• Target Species: Human
• Immunogen Description: STAT5B (NP_036580, 678 a.a. ~ 788 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Species: STAT5B antibody was raised against Human
• Antigen Type: Recombinant Protein
• Immunogen: STAT5B / STAT antibody was raised against sTAT5B (NP_036580, 678 a.a. ~ 788 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-STAT5B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 25 ug/ml])

Western Blot (WB)

(STAT5B monoclonal antibody clone 5D6 Western blot of STAT5B expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged STAT5B is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 21618344
• NCBI GeneID: 6777
• NCBI Accession #: NP_036580.2
• NCBI GenBank Nucleotide #: NM_012448.3
• UniProt Accession #: P51692; Q8WWS8
• Molecular Weight: 89,866 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5B
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5B
• NCBI Official Symbol: STAT5B
• NCBI Official Synonym Symbols: STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5B; transcription factor STAT5B
• UniProt Protein Name: Signal transducer and activator of transcription 5B
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5B
• UniProt Entry Name: STA5B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT5B: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases triggered by cytokines including IL2, IL3, GM-CSF, and various growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; double-stranded DNA binding; chromatin binding; protein phosphatase binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: lactation; transcription from RNA polymerase II promoter; succinate metabolic process; oxaloacetate metabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; response to lipopolysaccharide; progesterone metabolic process; female pregnancy; positive regulation of multicellular organism growth; fatty acid metabolic process; positive regulation of mitotic cell cycle; positive regulation of activated T cell proliferation; response to estradiol stimulus; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; allantoin metabolic process; natural killer cell differentiation; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; acute-phase response; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; positive regulation of natural killer cell proliferation; negative regulation of erythrocyte differentiation; citrate metabolic process; valine metabolic process; regulation of multicellular organism growth; development of secondary female sexual characteristics; positive regulation of cell motility; liver development; JAK-STAT cascade; positive regulation of natural killer cell mediated cytotoxicity; creatine metabolic process; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; response to ethanol; regulation of epithelial cell differentiation; response to hypoxia; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response; negative regulation of apoptosis

Disease: Growth Hormone Insensitivity With Immunodeficiency