Mouse anti-Human, Mouse TNFRSF11A / RANK Monoclonal Antibody | anti-TNFRSF11A antibody

Mouse Monoclonal [clone 9A725] (IgG1) to Human TNFRSF11A / RANK

Cat. Num. AAA245189

• Gene Names: TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: TNFRSF11A / RANK; Monoclonal Antibody; Mouse Monoclonal [clone 9A725] (IgG1) to Human TNFRSF11A / RANK; Anti-TNFRSF11A / RANK Antibody (aa326-616; clone 9A725) IHC-plus; TNFRSF11A; CD265; CD265 antigen; ODFR; LOH18CR1; OPTB7; OSTS; PDB2; RANK; Receptor activator of NF-KB; TRANCER; FEO; OFE; Human TNFRSF11A; anti-TNFRSF11A antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9A725
• Specificity: A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.2% gelatin, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 616

• Applicable Applications for anti-TNFRSF11A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo, IHC-P (10 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human TNFRSF11A / RANK.
• Immunogen Type: Recombinant protein
• Immunogen: TNFRSF11A / RANK antibody was raised against recombinant human TNFRSF11A / RANK.
• Antigen Modification: aa326-616
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TNFRSF11A / RANK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(Flow analysis of RANK in 10^6 RAW cells using 2 ug of antibody.  Shaded histogram represents RAW cells without antibody; green represents isotype control; red represents anti-RANK antibody.)

Western Blot (WB)

(Western blot of RANK in 293 cells transfected with RANK cDNA. A single protein band with an approximate mol. wt. of 97 kDa is detected.)

Product Categories/Family for anti-TNFRSF11A antibody

Family: TNF Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4507565
• NCBI GeneID: 8792
• NCBI Accession #: NP_003830.1
• NCBI GenBank Nucleotide #: NM_003839.3
• UniProt Accession #: Q9Y6Q6; Q59EP9; I4EC36; I4EC38; I4EC39; I7JE63; N0GVH0
• Molecular Weight: 64,552 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 11A isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: TNFRSF11A
• NCBI Official Synonym Symbols: FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A; Paget disease of bone 2; receptor activator of NF-KB; osteoclast differentiation factor receptor; receptor activator of nuclear factor-kappa B; loss of heterozygosity, 18, chromosomal region 1
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF11A
• UniProt Synonym Gene Names: RANK; ODFR
• UniProt Entry Name: TNR11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]

Uniprot Description

TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; tumor necrosis factor receptor activity; metal ion binding; cytokine binding; receptor activity

Biological Process: circadian thermoregulation; positive regulation of JNK activity; ossification; adaptive immune response; response to lipopolysaccharide; osteoclast differentiation; signal transduction; lymph node development; activation of NF-kappaB transcription factor; monocyte chemotaxis; response to radiation; tumor necrosis factor-mediated signaling pathway; cell-cell signaling; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of transcription factor activity

Disease: Osteopetrosis, Autosomal Recessive 7; Familial Expansile Osteolysis; Paget Disease Of Bone