Goat DAP12 Polyclonal Antibody | anti-DAP12 antibody

Goat Polyclonal to Human DAP12

Cat. Num. AAA244504

• Gene Names: TYROBP; DAP12; KARAP; PLOSL
• Reactivity: Bat, Gibbon, Chimpanzee, Dog, Gorilla, Horse, Human, Monkey; Predicted Reactivity: Rabbit, Sheep (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DAP12; Polyclonal Antibody; Goat Polyclonal to Human DAP12; Anti-DAP12 Antibody IHC-plus; TYROBP; DNAX-activation protein 12; PLOSL; KARAP; KAR-associated protein; PLO-SL; Human DAP12; anti-DAP12 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Chimpanzee, Dog, Gorilla, Horse, Human, Monkey; Predicted Reactivity: Rabbit, Sheep (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TYROBP / DAP12. This antibody is expected to recognize both reported isoforms (NP_003323.1; NP_937758.1)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 113

• Applicable Applications for anti-DAP12 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (3 - 6 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KQRITETESPYQE from an internal region of human TYROBP / DAP12 (NP_003323.1; NP_937758.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%); Sheep, Panda, Rabbit (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: DAP12 antibody was raised against synthetic peptide C-KQRITETESPYQE from an internal region of human TYROBP / DAP12 (NP_003323.1; NP_937758.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%); Sheep, Panda, Rabbit (92%); Mouse, Elephant, Zebu, Bovine (85%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TYROBP / DAP12 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TYROBP / DAP12 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507755
• NCBI GeneID: 7305
• NCBI Accession #: NP_003323.1
• NCBI GenBank Nucleotide #: NM_003332.3
• UniProt Accession #: O43914; Q6FGA5; Q9UMT3; A8K2X0; F5H389
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein isoform 1
• NCBI Official Synonym Full Names: TYRO protein tyrosine kinase binding protein
• NCBI Official Symbol: TYROBP
• NCBI Official Synonym Symbols: DAP12; KARAP; PLOSL
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein; KAR-associated protein; DNAX-activation protein 12; killer-activating receptor-associated protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: TYROBP
• UniProt Synonym Gene Names: DAP12; KARAP; KAR-associated protein
• UniProt Entry Name: TYOBP_HUMAN

NCBI Description

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; receptor signaling protein activity; receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; regulation of immune response; neutrophil activation during immune response; macrophage activation during immune response; innate immune response; cellular defense response; signal transduction

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy