Immunohistochemistry (IHC)
(Anti-NEK7 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Goat NEK7 Polyclonal Antibody | anti-NEK7 antibody
Goat Polyclonal to Human NEK7
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, RatPredicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
NEK7; Polyclonal Antibody; Goat Polyclonal to Human NEK7; Anti-NEK7 Antibody (Internal) IHC-plus; NimA-related protein kinase 7; NIMA-related kinase 7; Human NEK7; anti-NEK7 antibody
Host
Goat
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human NEK7.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
302
Applicable Applications for anti-NEK7 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 35kD band observed in lysates of cell line HeLa (calculated MW of 34.6kD according to NP_598001.1). Recommended concentration: 0.05-0.2 ug/ml.
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 35kD band observed in lysates of cell line HeLa (calculated MW of 34.6kD according to NP_598001.1). Recommended concentration: 0.05-0.2 ug/ml.
Target Species
Human
Immunogen Description
Synthetic peptide C-NTLANFRIEKK from an internal region of human NEK7 (NP_598001.1).
Immunogen Type
Synthetic peptide
Immunogen
NEK7 antibody was raised against synthetic peptide C-NTLANFRIEKK from an internal region of human NEK7 (NP_598001.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Xenopus (91%); Salmon, Stickleback, Pufferfish, Zebrafish (82%).
Antigen Modification
Internal
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Anti-NEK7 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Immunohistochemistry (IHC)
(Anti-NEK7 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Western Blot (WB)
(NEK7 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(NEK7 antibody (0.05 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-NEK7 antibody
NEK7 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain.
Product Categories/Family for anti-NEK7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,402 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek7
NCBI Official Synonym Full Names
NIMA-related kinase 7
NCBI Official Symbol
NEK7
NCBI Protein Information
serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7
UniProt Protein Name
Serine/threonine-protein kinase Nek7
Protein Family
UniProt Gene Name
NEK7
UniProt Synonym Gene Names
NimA-related protein kinase 7
UniProt Entry Name
NEK7_HUMAN
NCBI Description
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]
Uniprot Description
NEK7: a protein kinase of the NEK family. Phosphorylates and activates p70S6 kinase.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Other group; NEK family
Chromosomal Location of Human Ortholog: 1q31.3
Cellular Component: nucleoplasm; spindle pole; microtubule; cytoplasm; microtubule organizing center
Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding
Biological Process: regulation of mitotic cell cycle; regulation of signal transduction; cytokinesis; spindle assembly; protein amino acid phosphorylation
Research Articles on NEK7
1. Nek7, was significantly associated with certain clinicopathologic indices in gallbladder carcinoma
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Product Notes
The NEK7 nek7 (Catalog #AAA244158) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human NEK7 reacts with Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's NEK7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml) Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 35kD band observed in lysates of cell line HeLa (calculated MW of 34.6kD according to NP_598001.1). Recommended concentration: 0.05-0.2 ug/ml. Researchers should empirically determine the suitability of the NEK7 nek7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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