Immunohistochemistry (IHC)
(Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)
Rabbit anti-Human, Mouse Histone H2B Polyclonal Antibody | anti-HTB2 antibody
Rabbit Polyclonal (IgG) to Human Histone H2B
Gene Names
HTB2; H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
Histone H2B; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human Histone H2B; Anti-Histone H2B Antibody (aa10-59) IHC-plus; Human Histone H2B; anti-HTB2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Histone H2B (Ab-12) Antibody detects endogenous levels of total Histone H2B protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
145
Applicable Applications for anti-HTB2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:5000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Target Species
Human
Immunogen Description
Synthetic peptide from human Histone H2B (aa10-59).
Immunogen
Histone H2B antibody was raised against synthetic peptide from human Histone H2B (aa10-59).
Antigen Modification
aa10-59
Preparation and Storage
Store at -20 degree C for up to one year.
Immunohistochemistry (IHC)
(Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells, using Histone H2B (Ab-12) Antibody. The picture on the right is treated with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells, using Histone H2B (Ab-12) Antibody. The picture on the right is treated with the synthesized peptide.)
Western Blot (WB)
(Western blot of extracts from COS7 cells, treated with TSA 400 nM 24h, using Histone H2B (Ab-12) Antibody. The lane on the right is treated with the synthesized peptide.)
Western Blot (WB)
(Western blot of extracts from COS7 cells, treated with TSA 400 nM 24h, using Histone H2B (Ab-12) Antibody. The lane on the right is treated with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,732 Da
NCBI Official Full Name
histone H2B
NCBI Official Symbol
HTB2
NCBI Official Synonym Symbols
H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
NCBI Protein Information
histone H2B
UniProt Protein Name
Histone H2B.10
Protein Family
UniProt Gene Name
At5g22880
UniProt Entry Name
H2B10_ARATH
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Research Articles on HTB2
Similar Products
Product Notes
The HTB2 at5g22880 (Catalog #AAA244079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human Histone H2B reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA (1:5000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000). Researchers should empirically determine the suitability of the HTB2 at5g22880 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
