Rabbit anti-Human ANXA6/Annexin A6/Annexin VI Polyclonal Antibody | anti-ANXA6 antibody

Rabbit Polyclonal (IgG) to Human ANXA6/Annexin A6/Annexin VI

Cat. Num. AAA243708

• Gene Names: ANXA6; ANX6; CBP68
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ANXA6/Annexin A6/Annexin VI; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ANXA6/Annexin A6/Annexin VI; Anti-ANXA6/Annexin A6/Annexin VI Antibody (aa1-50) IHC-plus; ANXA6; 67 kDa calelectrin; Annexin VI (p68); Annexin A6; Annexin VI; ANX6; Calcium-binding protein p68; Calelectrin; CBP68; Chromobindin-20; Calphobindin-II; Lipocortin VI; p70; Protein III; Annexin-6; Calphobindin II; CPB-II; p68; Human ANXA6/Annexin A6/Annexin VI; anti-ANXA6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Annexin A6 Antibody detects endogenous levels of total Annexin A6 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-ANXA6 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Annexin A6 (aa1-50).
• Immunogen: ANXA6/Annexin A6/Annexin VI antibody was raised against synthetic peptide from human Annexin A6 (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-Annexin VI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using Annexin A6 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using Annexin A6 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-ANXA6 antibody

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kD that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths.

NCBI and Uniprot Product Information

• NCBI GI #: 71773329
• NCBI GeneID: 309
• NCBI Accession #: NP_001146.2
• NCBI GenBank Nucleotide #: NM_001155.4
• UniProt Accession #: P08133; Q6ZT79; B7Z8A7; D3DQH4; E9PGK1
• Molecular Weight: 72,423 Da
• NCBI Official Full Name: annexin A6 isoform 1
• NCBI Official Synonym Full Names: annexin A6
• NCBI Official Symbol: ANXA6
• NCBI Official Synonym Symbols: ANX6; CBP68
• NCBI Protein Information: annexin A6; p68; p70; CPB-II; annexin-6; calelectrin; lipocortin VI; calphobindin II; calphobindin-II; chromobindin-20; annexin VI (p68); 67 kDa calelectrin; calcium-binding protein p68
• UniProt Protein Name: Annexin A6
• Protein Family: Annexin
• UniProt Gene Name: ANXA6
• UniProt Synonym Gene Names: ANX6; CPB-II
• UniProt Entry Name: ANXA6_HUMAN

NCBI Description

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]

Uniprot Description

ANXA6: a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; membrane; perinuclear region of cytoplasm; late endosome membrane; lysosomal membrane; melanosome

Molecular Function: protein binding; protein homodimerization activity; GTP binding; calcium-dependent phospholipid binding; cholesterol binding; calcium ion binding; lipid binding; calcium-dependent protein binding; ligand-gated ion channel activity

Biological Process: regulation of muscle contraction; calcium ion transport; protein homooligomerization