Rabbit anti-Human IDE Polyclonal Antibody | anti-IDE antibody

Rabbit Polyclonal to Human IDE

Cat. Num. AAA243695

• Gene Names: IDE; INSULYSIN
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: IDE; Polyclonal Antibody; Rabbit Polyclonal to Human IDE; Anti-IDE Antibody IHC-plus; Abeta-degrading protease; Insulinase; INSULYSIN; Insulin protease; Insulin-degrading enzyme; Human IDE; anti-IDE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human insulin degrading enzyme. Predicted cross-reactivity based on amino acid sequence homology: mouse (94%), rat (95%), bovine (98%), zebrafish (90%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1019

• Applicable Applications for anti-IDE antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:5000 - 1:20000)
• Target Species: Human
• Immunogen Description: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 349 and 605 of Human insulin-degrading enzyme.
• Immunogen Type: Recombinant protein
• Immunogen: IDE antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 349 and 605 of Human insulin-degrading enzyme.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-IDE antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. D: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:10000.)

Related Product Information for anti-IDE antibody

Insulin Degrading Enzyme (IDE) encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of IDE for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid.

Product Categories/Family for anti-IDE antibody

Family: Protease; Subfamily: Metallopeptidase M16

NCBI and Uniprot Product Information

• NCBI GI #: 155969707
• NCBI GeneID: 3416
• NCBI Accession #: NP_004960.2
• NCBI GenBank Nucleotide #: NM_004969.3
• UniProt Accession #: P14735; Q5T5N2; B2R721; B7ZAU2; D3DR35
• Molecular Weight: 54,240 Da
• NCBI Official Full Name: insulin-degrading enzyme isoform 1
• NCBI Official Synonym Full Names: insulin-degrading enzyme
• NCBI Official Symbol: IDE
• NCBI Official Synonym Symbols: INSULYSIN
• NCBI Protein Information: insulin-degrading enzyme; insulinase; insulin protease; Abeta-degrading protease
• UniProt Protein Name: Insulin-degrading enzyme
• Protein Family: IgM protease
• UniProt Gene Name: IDE
• UniProt Synonym Gene Names: Insulinase
• UniProt Entry Name: IDE_HUMAN

NCBI Description

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]

Uniprot Description

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Nuclear receptor co-regulator; Cell surface; EC 3.4.24.56

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: nucleoplasm; peroxisomal matrix; extracellular space; cell surface; mitochondrion; cytoplasm; plasma membrane; peroxisome; nucleus; cytosol

Molecular Function: insulin binding; ubiquitin binding; protein binding; protein homodimerization activity; zinc ion binding; metalloendopeptidase activity; beta-amyloid binding; ATPase activity; glycoprotein binding; peptide binding; beta-endorphin binding; ATP binding; receptor binding

Biological Process: negative regulation of proteolysis; positive regulation of protein oligomerization; proteolysis involved in cellular protein catabolic process; viral reproduction; protein heterooligomerization; beta-amyloid metabolic process; insulin receptor signaling pathway; hormone catabolic process; determination of adult life span; proteolysis; protein homooligomerization; protein homotetramerization