Goat G6PD Polyclonal Antibody | anti-G6PD antibody

Goat Polyclonal to Human G6PD

Cat. Num. AAA242686

• Gene Names: G6PD; G6PD1
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey; Predicted Reactivity: Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Sheep, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: G6PD; Polyclonal Antibody; Goat Polyclonal to Human G6PD; Anti-G6PD Antibody (aa305-318) IHC-plus; G6PD1; Human G6PD; anti-G6PD antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey; Predicted Reactivity: Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Sheep, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human G6PD. This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 545

• Applicable Applications for anti-G6PD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (2.5 ug/ml), WB (1:8000, 0.1 - 0.3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KPASTNSDDVRDEK from an internal region of human G6PD (NP_000393.4 ; NP_001035810.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Opossum, Platypus, Lizard, Pufferfish (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: G6PD antibody was raised against synthetic peptide C-KPASTNSDDVRDEK from an internal region of human G6PD (NP_000393.4 ; NP_001035810.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Opossum, Platypus, Lizard, Pufferfish (100%); Galago, Rat, Sheep, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Horse, Xenopus, Trout, Salmon, Stickleback, Zebrafish (93%); Mouse, Shrew, Cat, Medaka (86%).
• Antigen Modification: aa305-318
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-G6PD antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-G6PD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 109389365
• NCBI GeneID: 2539
• NCBI Accession #: NP_000393.4
• NCBI GenBank Nucleotide #: NM_000402.4
• UniProt Accession #: P11413; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
• Molecular Weight: 62,468 Da
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase isoform a
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6PD
• NCBI Official Synonym Symbols: G6PD1
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6PD
• UniProt Synonym Gene Names: G6PD
• UniProt Entry Name: G6PD_HUMAN

NCBI Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Oxidoreductase; Other Amino Acids Metabolism - glutathione; EC 1.1.1.49; Cell development/differentiation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; glucose-6-phosphate dehydrogenase activity; protein homodimerization activity; NADP binding; glucose binding

Biological Process: response to food; NADPH regeneration; pentose-phosphate shunt; ribose phosphate biosynthetic process; erythrocyte maturation; glucose 6-phosphate metabolic process; pentose-phosphate shunt, oxidative branch; glucose metabolic process; cytokine production; pathogenesis; NADP metabolic process; response to organic cyclic substance; cholesterol biosynthetic process; response to ethanol; substantia nigra development; glutathione metabolic process; regulation of neuron apoptosis; carbohydrate metabolic process; lipid metabolic process; pentose biosynthetic process

Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To