Goat CENPF / CENP-F Polyclonal Antibody | anti-CENPF antibody

Goat Polyclonal to Human CENPF / CENP-F

Cat. Num. AAA242630

• Gene Names: CENPF; CENF; hcp-1; PRO1779
• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CENPF / CENP-F; Polyclonal Antibody; Goat Polyclonal to Human CENPF / CENP-F; Anti-CENPF / CENP-F Antibody (C-Terminus) IHC-plus; CENPF; AH antigen; Centromere protein F; CENF; CENP-F; Kinetochore protein CENPF; Mitosin; CENP-F kinetochore protein; Hcp-1; PRO1779; Human CENPF; anti-CENPF antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CENPF. The immunizing peptide shows one mismatch with the corresponmding sequence of the NCBI-curated isoform NP_057427.3.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 3114

• Applicable Applications for anti-CENPF antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide GLESKGSENCKVQ from the C-terminus of human CENPF (AAA82889.1; AAA82935.1; AAA86889.1). Percent identity by BLAST analysis: Gorilla (100%); Human, Monkey (92%); Gibbon, Bovine (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: CENPF / CENP-F antibody was raised against synthetic peptide GLESKGSENCKVQ from the C-terminus of human CENPF (AAA82889.1; AAA82935.1; AAA86889.1). Percent identity by BLAST analysis: Gorilla (100%); Human, Monkey (92%); Gibbon, Bovine (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CENPF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 55770834
• NCBI GeneID: 1063
• NCBI Accession #: NP_057427.3
• NCBI GenBank Nucleotide #: NM_016343.3
• UniProt Accession #: P49454; Q13171; Q13246; Q5VVM7
• Molecular Weight: 367,764 Da
• NCBI Official Full Name: centromere protein F
• NCBI Official Synonym Full Names: centromere protein F, 350/400kDa
• NCBI Official Symbol: CENPF
• NCBI Official Synonym Symbols: CENF; hcp-1; PRO1779
• NCBI Protein Information: centromere protein F; mitosin; AH antigen; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin)
• UniProt Protein Name: Centromere protein F
• Protein Family: Centromere protein
• UniProt Gene Name: CENPF
• UniProt Synonym Gene Names: CENP-F
• UniProt Entry Name: CENPF_HUMAN

NCBI Description

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPF: a nuclear matrix protein that assembles onto kinetochores at late G2. Is hyperphosphorylated during mitosis and rapidly degraded after mitosis. Involved in chromosome segregation during mitosis. Interacts with retinoblastoma protein (RB), CENP-E and BUBR1. Two alternatively spliced human isoforms have been described.

Protein type: Cell cycle regulation; Transcription, coactivator/corepressor; Nuclear envelope

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: spindle pole; nuclear matrix; pronucleus; nuclear envelope; cytosol; kinetochore; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; outer kinetochore of condensed chromosome; spindle; midbody; nucleus; chromatin; chromosome, pericentric region

Molecular Function: protein C-terminus binding; dynein binding; protein binding; protein homodimerization activity; chromatin binding; transcription factor binding

Biological Process: response to drug; mitosis; muscle development; kinetochore assembly; regulation of cell cycle; chromosome segregation; cell proliferation; protein transport; cell division; mitotic cell cycle spindle assembly checkpoint; regulation of G2/M transition of mitotic cell cycle; metaphase plate congression; mitotic cell cycle; cell differentiation; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of striated muscle development

Disease: Ciliary Dyskinesia, Primary, 31