Goat MAOB / Monoamine Oxidase B Polyclonal Antibody | anti-MAOB antibody

Goat Polyclonal to Human MAOB / Monoamine Oxidase B

Cat. Num. AAA242608

• Reactivity: Gibbon, Bovine, Guinea pig, Gorilla, Horse, Human, Monkey, Orangutan, Pig, Rabbit; Predicted Reactivity: Mouse, Rat, Dog (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MAOB / Monoamine Oxidase B; Polyclonal Antibody; Goat Polyclonal to Human MAOB / Monoamine Oxidase B; Anti-MAOB / Monoamine Oxidase B Antibody (Internal) IHC-plus; MAOB; Adrenalin oxidase; MAO; brain; MAO-B; Monoamine oxidase type B; platelet; Tyramine oxidase; Monoamine oxidase B; Human MAOB; Monoamine Oxidase B; anti-MAOB antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Guinea pig, Gorilla, Horse, Human, Monkey, Orangutan, Pig, Rabbit; Predicted Reactivity: Mouse, Rat, Dog (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MAOB. This antibody is not expected to cross-react with MAOA.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 520

• Applicable Applications for anti-MAOB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-HKARKLARLTKEE from an internal region of human MAOB (NP_000889.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: MAOB / Monoamine Oxidase B antibody was raised against synthetic peptide C-HKARKLARLTKEE from an internal region of human MAOB (NP_000889.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Mouse, Rat, Panda, Dog (92%); Bat (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAOB antibody IHC of human brain. cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 38202207
• NCBI GeneID: 4129
• NCBI Accession #: NP_000889.3
• NCBI GenBank Nucleotide #: NM_000898.4
• UniProt Accession #: P27338; Q7Z6S2; B2R6R3; B7Z5H3; D3DWC3
• Molecular Weight: 46,539 Da
• NCBI Official Full Name: amine oxidase
• NCBI Official Synonym Full Names: monoamine oxidase B
• NCBI Official Symbol: MAOB
• NCBI Protein Information: amine oxidase [flavin-containing] B; amine oxidase [flavin-containing] B; MAO-B; MAO, brain; MAO, platelet; tyramine oxidase; adrenalin oxidase; monoamine oxidase type B
• UniProt Protein Name: Amine oxidase [flavin-containing] B
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOB
• UniProt Synonym Gene Names: MAO-B
• UniProt Entry Name: AOFB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq, Jul 2008]

Uniprot Description

MAOB: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. Belongs to the flavin monoamine oxidase family.

Protein type: Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Mitochondrial; Amino Acid Metabolism - tyrosine; Oxidoreductase; Membrane protein, integral; EC 1.4.3.4; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: mitochondrial envelope; mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; FAD binding; electron carrier activity

Biological Process: response to drug; positive regulation of dopamine metabolic process; negative regulation of serotonin secretion; response to selenium ion; substantia nigra development; response to ethanol; dopamine catabolic process; response to toxin; xenobiotic metabolic process; response to lipopolysaccharide; hydrogen peroxide biosynthetic process; response to aluminum ion; response to corticosterone stimulus