Goat GPX3 Polyclonal Antibody | anti-GPX3 antibody

Goat Polyclonal to Human GPX3

Cat. Num. AAA242329

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rat; Predicted Reactivity: Rabbit, Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GPX3; Polyclonal Antibody; Goat Polyclonal to Human GPX3; Anti-GPX3 Antibody (Internal) IHC-plus; Glutathione peroxidase 3; GSHPx-3; GPXP; GSHPx-P; GPx-3; GPx-P; Plasma glutathione peroxidase; Human GPX3; anti-GPX3 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rat; Predicted Reactivity: Rabbit, Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPX3. This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 226

• Applicable Applications for anti-GPX3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPX3 antibody was raised against synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%); Rabbit, Opossum, Chicken (92%); Hamster, Zebrafish, Pseudomonas (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-GPX3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GPX3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-GPX3 antibody

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme. GPX3 expression appears to be tissue-specific.

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3 (plasma)
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Oxidoreductase; Secreted; Secreted, signal peptide; Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; response to lipid hydroperoxide; hydrogen peroxide catabolic process; protein homotetramerization