Immunohistochemistry
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with PPARG antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Human Peroxisome Proliferator-Activated Receptor Gamma (PPARG) Polyclonal Antibody | anti-PPARG antibody
PPARG Rabbit anti-Human Polyclonal (aa82-101) Antibody
Gene Names
PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunoaffinity Chromatography
Synonyms
Peroxisome Proliferator-Activated Receptor Gamma (PPARG); Polyclonal Antibody; PPARG Rabbit anti-Human Polyclonal (aa82-101) Antibody; PPARG; CIMT1; GLM1; NR1C3; Peroxisome proliferator activated receptor gamma; PPAR gamma 2; PPAR-gamma; Ppar gamma; PPARG1; PPARG2; PPARgamma; Ppargamma 2; anti-PPARG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human PPAR gamma 1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE; amino acids 110-129 of PPAR gamma 2)1
Purity/Purification
Immunoaffinity Chromatography
Form/Format
TBS, 50% glycerol, 0.5 mg/ml BSA, 0.02% sodium azide.
Concentration
0.6 mg/ml (varies by lot)
Applicable Applications for anti-PPARG antibody
Western Blot, Immunohistochemistry - Paraffin
Application Notes
Immunohistochemistry - Paraffin (IHC-P) (5 ug/ml), Western blot (WB) (Optimal dilution to be determined by the researcher)
Antibody Validation: It has been validated for use in the following assays: IHC.
Antibody Usage Summary: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 5 ug/ml.
Antibody Validation: It has been validated for use in the following assays: IHC.
Antibody Usage Summary: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 5 ug/ml.
Immunogen
PPARG antibody was raised against synthetic peptide
Antigen Modification
aa82-101
Antigen Type
Synthetic peptide - KLH conjugated
Antigen Species
PPARG antibody was raised against Human
Predicted Species Reactivity
Mouse
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C
Immunohistochemistry
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with PPARG antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with PPARG antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)
Product Categories/Family for anti-PPARG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,620 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma isoform 2
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor gamma
NCBI Official Symbol
PPARG
NCBI Official Synonym Symbols
GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
NCBI Protein Information
peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; OTTHUMP00000160185; OTTHUMP00000160186; OTTHUMP00000185030; OTTHUMP00000185032; OTTHUMP00000185034; OTTHUMP00000185036; OTTHUMP00000207750; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated nuclear receptor gamma variant 1
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma
Protein Family
UniProt Gene Name
PPARG
UniProt Synonym Gene Names
NR1C3
UniProt Entry Name
PPARG_HUMAN
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Product Notes
The PPARG pparg (Catalog #AAA241622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPARG Rabbit anti-Human Polyclonal (aa82-101) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxisome Proliferator-Activated Receptor Gamma (PPARG) can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry - Paraffin. Immunohistochemistry - Paraffin (IHC-P) (5 ug/ml), Western blot (WB) (Optimal dilution to be determined by the researcher) Antibody Validation: It has been validated for use in the following assays: IHC. Antibody Usage Summary: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 5 ug/ml. . Researchers should empirically determine the suitability of the PPARG pparg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferator-Activated Receptor Gamma (PPARG), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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