Human Matrix Gla Protein ELISA Kit | MGP elisa kit

Human Matrix Gla Protein ELISA Kit

Cat. Num. AAA24150

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Human
• Synonyms: Matrix Gla Protein; Human Matrix Gla Protein ELISA Kit; MGP elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human MGP and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml - 200 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MGP concentrations in Human serum, plasma and other body fluids. Using Purified Human MGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGP and MGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MGP elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MGP concentrations in Human serum, plasma and other body fluids. Using Purified Human MGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGP and MGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 187591
• NCBI GeneID: 4256
• UniProt Accession #: P08493; Q2TU41; Q567P9; Q6ICN5; A0M8W5; B2R519; J3KMX7
• Molecular Weight: 12,353 Da
• NCBI Official Full Name: matrix Gla protein
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein; cell growth-inhibiting gene 36 protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; MGP
• UniProt Entry Name: MGP_HUMAN

NCBI Description

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding

Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation

Disease: Keutel Syndrome