Rabbit CDK9 Polyclonal Antibody | anti-CDK9 antibody

Rabbit Polyclonal to Human CDK9

Cat. Num. AAA240589

• Gene Names: CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Delipidated and defibrinated
• Synonyms: CDK9; Polyclonal Antibody; Rabbit Polyclonal to Human CDK9; Anti-CDK9 Antibody IHC-plus; C-2k; CDC2L4; CDC2-related kinase; Cell division protein kinase 9; Cyclin-dependent kinase 9; CDC2-related protein kinase; PITALRE; TAK; CTK1; Human CDK9; anti-CDK9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
• Purity/Purification: Delipidated and defibrinated
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Sequence Length: 372

• Applicable Applications for anti-CDK9 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), ICC, IHC-P (1:500), IP, WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CDK9.
• Immunogen Type: Synthetic peptide
• Immunogen: CDK9 antibody was raised against synthetic peptide from human CDK9.
• Preparation and Storage: Store vial at -20 degree C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CDK9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Immunocytochemistry (ICC)

(Anti-cdk9 (PITALRE) Antibody - Immunocytochemical Staining. Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Sections from each specimen were cut at 5-7 micron, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h with rabbit anti-cdk9 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C. Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain.)

Western Blot (WB)

(Anti-cdk9 (PITALRE) Antibody - Western blot. Anti-cdk9 antibody (1:1500) was used for Western blot of 1) PC3 and 2) DU145 prostate cancer cells (50 ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University.)

Related Product Information for anti-CDK9 antibody

CDK9, a CDC2/CDK type protein kinase, is a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription. CDK9 associates with cyclin K or T, and this complex phosphorylates the C-terminal domain (CTD) of the holoenzyme. CDK9/cyclin K also has been shown to phosphorylate the retinoblastoma protein (Rb) and myelin basic protein. At least two mRNA transcripts have been reported -- 2.8- and 4.3-kb.

Product Categories/Family for anti-CDK9 antibody

Family: Protein Kinase; Subfamily: CDC2/CDK

NCBI and Uniprot Product Information

• NCBI GI #: 4502747
• NCBI GeneID: 1025
• NCBI Accession #: NP_001252.1
• NCBI GenBank Nucleotide #: NM_001261.3
• UniProt Accession #: P50750; Q5JU24; Q5JU25; Q5U006; Q96TF1
• Molecular Weight: 53,365 Da
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin-dependent kinase 9
• NCBI Official Symbol: CDK9
• NCBI Official Synonym Symbols: TAK; C-2k; CTK1; CDC2L4; PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9; CDC2-related kinase; cell division protein kinase 9; serine/threonine protein kinase PITALRE; cell division cycle 2-like protein kinase 4; tat-associated kinase complex catalytic subunit
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK9
• UniProt Synonym Gene Names: CDC2L4; TAK
• UniProt Entry Name: CDK9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: EC 2.7.11.23; Kinase, protein; Cell cycle regulation; EC 2.7.11.22; Protein kinase, CMGC; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of histone phosphorylation; viral reproduction; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; replication fork arrest; positive regulation of transcription from RNA polymerase II promoter