Rabbit anti-Mouse GLI2 Polyclonal Antibody | anti-GLI2 antibody

Rabbit Polyclonal to Mouse GLI2

Cat. Num. AAA240221

• Gene Names: GLI2; CJS; HPE9; PHS2; THP1; THP2
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GLI2; Polyclonal Antibody; Rabbit Polyclonal to Mouse GLI2; Anti-GLI2 Antibody (Internal) IHC-plus; GLI-Kruppel family member GLI2; HPE9; Gli-2; Oncogene GLI2; Tax helper protein 2; THP; Zinc finger protein GLI2; Tax helper protein; THP2; GLI family zinc finger 2; Tax helper protein 1; THP1; Mouse GLI2; anti-GLI2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Amino acids from an internal region of Mouse Gli-2.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1586

• Applicable Applications for anti-GLI2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:15000 - 1:60000), IHC-P (5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Mouse
• Immunogen Description: Synthetic peptide from mouse GLI2.
• Immunogen Type: Synthetic peptide
• Immunogen: GLI2 antibody was raised against synthetic peptide from mouse GLI2.
• Antigen Modification: Internal
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-GLI2 antibody IHC of mouse, testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GLI2 antibody IHC of mouse, brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western analysis of mouse brain whole lysate.)

Related Product Information for anti-GLI2 antibody

Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 86991432
• NCBI GeneID: 2736
• NCBI Accession #: NP_005261.2
• NCBI GenBank Nucleotide #: NM_005270.4
• UniProt Accession #: P10070; O60252; O60253; O60254; O60255; Q15590; Q15591; Q4JHT4
• Molecular Weight: 86,482 Da
• NCBI Official Full Name: zinc finger protein GLI2
• NCBI Official Synonym Full Names: GLI family zinc finger 2
• NCBI Official Symbol: GLI2
• NCBI Official Synonym Symbols: CJS; HPE9; PHS2; THP1; THP2
• NCBI Protein Information: zinc finger protein GLI2; oncogene GLI2; tax helper protein 1; tax helper protein 2; GLI-Kruppel family member GLI2; GLI family zinc finger protein 2; tax-responsive element-2 holding protein; glioma-associated oncogene family zinc finger 2; tax-responsive element-25-bp sequence binding protein
• UniProt Protein Name: Zinc finger protein GLI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: GLI2
• UniProt Entry Name: GLI2_HUMAN

NCBI Description

This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).