Immunohistochemistry (IHC)
(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human DLK1/Pref-1 Monoclonal Antibody | anti-DLK1/Pref-1 antibody
Anti-DLK1/Pref-1 Antibody (Extracellular Domain, clone PF299-1)
FC/FACS: 1:100-1:500
IHC-P: 10 ug/ml
WB: 1:2000-1:10000
Long term: -20 degree C.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (WB)
(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis of human Pref-1using anti-DLK1 (human), mAb (PF299-1) at 1: 2, 000 dilution. A.. 1. Human Pref-1 Fc protein. 2. Transfected human Pref-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B.. 1. Human Pref-1 Fc protein. 2. Mouse Pref-1 Fc protein. 3. Human DNER Fc protein. 4. Human DLL1 Fc protein. 5. Human DLL3 Fc protein. 6. Human DLL4 Fc protein. 7. Human Jagged1 Fc protein. 8. Human FGF23 Fc protein.)
Western Blot (WB)
(Western blot analysis of human Pref-1using anti-DLK1 (human), mAb (PF299-1) at 1: 2, 000 dilution. A.. 1. Human Pref-1 Fc protein. 2. Transfected human Pref-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. B.. 1. Human Pref-1 Fc protein. 2. Mouse Pref-1 Fc protein. 3. Human DNER Fc protein. 4. Human DLL1 Fc protein. 5. Human DLL3 Fc protein. 6. Human DLL4 Fc protein. 7. Human Jagged1 Fc protein. 8. Human FGF23 Fc protein.)
Flow Cytometry (FC/FACS)
(FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF299-1).)
Flow Cytometry (FC/FACS)
(FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF299-1).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]
Uniprot Description
May have a role in neuroendocrine differentiation.
Research Articles on DLK1/Pref-1
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Product Notes
The DLK1/Pref-1 dlk1 (Catalog #AAA2401788) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-DLK1/Pref-1 Antibody (Extracellular Domain, clone PF299-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLK1/Pref-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:2000-1:10000 FC/FACS: 1:100-1:500 IHC-P: 10 ug/ml WB: 1:2000-1:10000. Researchers should empirically determine the suitability of the DLK1/Pref-1 dlk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLK1/Pref-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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