Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit POLK/DNA Polymerase Kappa Polyclonal Antibody | anti-POLK antibody
Anti-POLK/DNA Polymerase Kappa Antibody
Predicted: Mouse, Rat
Predicted: Mouse, Rat
IHC: 1:50-1:200
IHC-P: 1:100
WB: 1:500-1:2000
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cells.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using POLK antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using POLK antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using POLK antibody. Green[Character ff1a]GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser as they always gather around DNA damage region.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using POLK antibody. Green[Character ff1a]GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser as they always gather around DNA damage region.)
Western Blot (WB)
(Western blot analysis of extracts of various cells.)
Western Blot (WB)
(Western blot analysis of extracts of various cells.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
Uniprot Description
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Research Articles on POLK
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Product Notes
The POLK polk (Catalog #AAA2401531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-POLK/DNA Polymerase Kappa Antibody reacts with Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLK/DNA Polymerase Kappa can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:50-1:200 IHC: 1:50-1:200 IHC-P: 1:100 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the POLK polk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLK/DNA Polymerase Kappa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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