Rabbit anti-Human PIP5K1A Polyclonal Antibody | anti-PIP5K1A antibody

Anti-PIP5K1A Antibody (Internal)

Cat. Num. AAA2401004

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PIP5K1A; Polyclonal Antibody; Anti-PIP5K1A Antibody (Internal); Rabbit Polyclonal to Human PIP5K1A; PIP5KIalpha; PtdIns(4)P-5-kinase 1 alpha; PIP5K1-alpha; anti-PIP5K1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: BLAST analysis suggests cross-reactivity with mouse based on 100% sequence homology.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 30% glycerol, sterile filtered
• Sequence Length: 549

• Applicable Applications for anti-PIP5K1A antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:10000; IHC-P: 5 ug/ml; WB: 1 ug/ml
• Immunogen: Anti-PI4K, type I alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein.
• Conjugation: Unconjugated
• Epitope: Internal
• Preparation and Storage: Short term storage: 4 degree C; Long term storage: aliquot and store at -20 degree C, avoid freeze thaw cycles.; Centrifuge product before removing cap. Only dilute immediately prior to use.

Immunohistochemistry (IHC)

(Anti-PIP5K1A antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-PIP5K1A antibody

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate(PtdIns4P) to form phosphatidylinositol 4, 5-bisphosphate(PtdIns(4, 5)P2). PtdIns(4, 5)P2 is involved in a variety of cellularprocesses and is the substrate to form phosphatidylinositol3, 4, 5-trisphosphate (PtdIns(3, 4, 5)P3), another second messenger. the majority of PtdIns(4, 5)P2 is thought to occur via type Iphosphatidylinositol 4-phosphate 5-kinases given the abundance ofPtdIns4P.

Product Categories/Family for anti-PIP5K1A antibody

Non-Protein Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4505815
• NCBI GeneID: 8394
• NCBI Accession #: NP_003548.1
• NCBI GenBank Nucleotide #: NM_003557.2
• UniProt Accession #: Q99755; Q99754; Q99756; A8K4Q0; B4DIN0
• Molecular Weight: 58,119 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 2
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
• NCBI Official Symbol: PIP5K1A
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1A
• UniProt Synonym Gene Names: PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha

Uniprot Description

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.