Western Blot (WB)
(Anti-GPR175 Antibody at 1:1000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GPR175 Polyclonal Antibody | anti-TPRA1 antibody
GPR175 Antibody
Gene Names
TPRA1; GPR175; TPRA40; TMEM227
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GPR175; Polyclonal Antibody; GPR175 Antibody; Transmembrane protein adipocyte-associated 1; Integral membrane protein GPR175; Transmembrane protein 227; TPRA1; TMEM227; anti-TPRA1 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
171-230
Sequence Length
373
Applicable Applications for anti-TPRA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human GPR175
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt
Western Blot (WB)
(Anti-GPR175 Antibody at 1:1000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GPR175 Antibody at 1:1000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Product Categories/Family for anti-TPRA1 antibody
References
Saito T.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Yang H.,et al.Endocrinology 140:2859-2867(1999).
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Yang H.,et al.Endocrinology 140:2859-2867(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41053
NCBI Official Full Name
transmembrane protein adipocyte-associated 1 isoform 1
NCBI Official Synonym Full Names
transmembrane protein, adipocyte asscociated 1
NCBI Official Symbol
TPRA1
NCBI Official Synonym Symbols
GPR175; TPRA40; TMEM227
NCBI Protein Information
transmembrane protein adipocyte-associated 1
UniProt Protein Name
Transmembrane protein adipocyte-associated 1
Protein Family
UniProt Gene Name
TPRA1
UniProt Synonym Gene Names
GPR175; TMEM227
UniProt Entry Name
TPRA1_HUMAN
Uniprot Description
GPR175: Belongs to the UPF0359 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 3q21.2
Cellular Component: integral to membrane
Molecular Function: G-protein coupled receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; lipid metabolic process; aging
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Product Notes
The TPRA1 tpra1 (Catalog #AAA9211554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 171-230. The GPR175 Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPR175 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~ 1:1000. Researchers should empirically determine the suitability of the TPRA1 tpra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR175, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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