Rabbit GPC1/Glypican Polyclonal Antibody | anti-GPC1 antibody

Anti-GPC1/Glypican Antibody (Internal)

Cat. Num. AAA2400985

• Gene Names: GPC1; glypican
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Protein A purified
• Synonyms: GPC1/Glypican; Polyclonal Antibody; Anti-GPC1/Glypican Antibody (Internal); Mouse Monoclonal (IgG1; k) to Human GPC1/Glypican; GPC1; Glypican 1; Glypican; Glypican-1; Glypican proteoglycan 1; anti-GPC1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
• Purity/Purification: Protein A purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Sequence Length: 558

• Applicable Applications for anti-GPC1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:10000-1:50000; IHC-P: 10 ug/ml; WB: 1:500-1:2000
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
• Conjugation: Unconjugated
• Epitope: Internal
• Preparation and Storage: Short Term: Store at 4 degree C; Long Term: Aliquot and store at -20 degree C; Avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-GPC1/Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Anti-Glypican-1 Antibody - Western Blot. Western blot of protein A purified anti-Glypican-1 antibody shows detection of exogenous glypican-1 in 50 ug 293T whole cell lysate (lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1:1000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1:5000 in 5% BLOTTO at 4C. Lane 1: untransfected 293T cell lysate. Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD.)

Related Product Information for anti-GPC1 antibody

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates inSchwann cell myelination (By similarity). May act as a catalyst inincreasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc)via associating (via the heparan sulfate side chains) with both forms ofPRPN, targeting them to lipid rafts and facilitating their interaction.

NCBI and Uniprot Product Information

• NCBI GI #: 167001141
• NCBI GeneID: 2817
• NCBI Accession #: NP_002072.2
• NCBI GenBank Nucleotide #: NM_002081.2
• UniProt Accession #: P35052; Q53QM4; B3KTD1
• Molecular Weight: 31,931 Da
• NCBI Official Full Name: glypican-1
• NCBI Official Synonym Full Names: glypican 1
• NCBI Official Symbol: GPC1
• NCBI Official Synonym Symbols: glypican
• NCBI Protein Information: glypican-1
• UniProt Protein Name: Glypican-1
• Protein Family: Glypican
• UniProt Gene Name: GPC1

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.