TMB CORE Accessory Reagent | TMB accessory reagent
TMB CORE
Applications
ELISA
Synonyms
TMB CORE; TMB accessory reagent
Form/Format
Ready To Use
Ready to use TMB solution - liquid
Ready to use TMB solution - liquid
Applicable Applications for TMB accessory reagent
ELISA (EIA)
Application Notes
ELISA: Maximum Dilution: Neat
Intended Use
This item is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).
TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution.
This item produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Instructions For Use
TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution.
This item produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Instructions For Use
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product is photosensitive and should be protected from light. Avoid exposure to heat and contamination with metal ions or peroxidase. Store in bottles made of High Density Polyethylene (HDPE).
Shelf Life: 24 months from date of despatch.
Shelf Life: 24 months from date of despatch.
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Product Notes
The TMB (Catalog #AAA239088) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TMB CORE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the TMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMB CORE, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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