Human Cytotoxic T-Lymphocyte Associated Antigen 4 ELISA Kit | CTLA4 elisa kit

Human Cytotoxic T-Lymphocyte Associated Antigen 4 ELISA Kit

Cat. Num. AAA23839

• Gene Names: CTLA4; CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3
• Reactivity: Human
• Synonyms: Cytotoxic T-Lymphocyte Associated Antigen 4; Human Cytotoxic T-Lymphocyte Associated Antigen 4 ELISA Kit; CTLA4 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human CTLA4 and analogues was observed.
• Sequence Length: 223

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CTLA4 concentrations in Human serum, plasma and other body fluids. Using Purified Human CTLA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTLA4 and CTLA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTLA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CTLA4 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CTLA4 concentrations in Human serum, plasma and other body fluids. Using Purified Human CTLA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTLA4 and CTLA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTLA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361212
• NCBI GeneID: 1493
• NCBI Accession #: NP_005205.2
• NCBI GenBank Nucleotide #: NM_005214.4
• UniProt Accession #: P16410; O95653; Q0PP65; Q52MC1; Q53TD5; Q5S005; Q8WXJ1; Q96P43; Q9UKN9; A0N1S0; E9PDH0
• Molecular Weight: 24,656 Da
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte-associated protein 4
• NCBI Official Symbol: CTLA4
• NCBI Official Synonym Symbols: CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; insulin-dependent diabetes mellitus 12; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• Protein Family: Cytotoxic T-lymphocyte protein
• UniProt Gene Name: CTLA4
• UniProt Synonym Gene Names: CD152; CTLA-4
• UniProt Entry Name: CTLA4_HUMAN

NCBI Description

This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Molecular Function: protein binding

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of apoptosis; negative regulation of regulatory T cell differentiation; T cell costimulation; negative regulation of immune response; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus

Disease: Autoimmune Lymphoproliferative Syndrome, Type V; Celiac Disease, Susceptibility To, 3; Diabetes Mellitus, Insulin-dependent, 12; Systemic Lupus Erythematosus; Hashimoto Thyroiditis