Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC .)
Mouse anti-Pig SLA CLASS II DQ Monoclonal Antibody | anti-SLA Class II DQ antibody
MOUSE ANTI PIG SLA CLASS II DQ
Reactivity
Pig
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Synonyms
SLA CLASS II DQ; Monoclonal Antibody; MOUSE ANTI PIG SLA CLASS II DQ; SLA Class II DQ antibody|K274.3G8; anti-SLA Class II DQ antibody
Host
Mouse
Reactivity
Pig
Clonality
Monoclonal
Isotype
IgG1
Clone Number
K274.3G8
Specificity
SLA CLASS II DQ
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A
Purified IgG prepared by affinity chromatography on Protein A
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-SLA Class II DQ antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)-Frozen, Immunohistochemistry (IHC)-Paraffin
Application Notes
FC/FACS: 1/10-1/100
FC/FACS: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
FC/FACS: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Species Cross-Reactivity
Bovine
Immunogen
Porcine peripheral blood lymphocytes
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3-X63-Ag.653 myeloma cell line
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC .)
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC .)
Related Product Information for anti-SLA Class II DQ antibody
Mouse anti Pig SLA Class II DQ antibody, clone K274.3G8 recognizes SLA DQ molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.
References
1. Lunney, J.K. (1993) Characterization of swine leukocyte differentiation antigens.Immunol Today 14: 147-8.
2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.Vet Immunol Immunopathol. 64: 1-13.
3. Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.Vet Pathol. 40: 395-404.
4. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88: 571-8.
5. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.J Pharmacol Exp Ther. 302: 861-70.
2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.Vet Immunol Immunopathol. 64: 1-13.
3. Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.Vet Pathol. 40: 395-404.
4. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88: 571-8.
5. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.J Pharmacol Exp Ther. 302: 861-70.
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Product Notes
The SLA Class II DQ (Catalog #AAA225941) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG SLA CLASS II DQ reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SLA CLASS II DQ can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)-Frozen, Immunohistochemistry (IHC)-Paraffin. FC/FACS: 1/10-1/100 FC/FACS: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the SLA Class II DQ for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLA CLASS II DQ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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